positive control for apoptosis

[Martin Wessendorf-2]

Dear all--

Can anyone suggest a good tissue to use as a positive control for
apoptosis?  We've considered liver and testes but as nearly as I can
find from the literature, the proportion of apoptotic cells in those
organs in naive rats is pretty small.  Any other suggestions?

Thanks!

Martin Wessendorf
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]

[Patricia Masso-Welch]

Untreated thymus is a great tissue.

Patty Welch

--On Friday, July 10, 2009 12:58 PM -0500 Martin Wessendorf
<[hidden email]> wrote:

Dear all--

Can anyone suggest a good tissue to use as a positive control for
apoptosis?  We've considered liver and testes but as nearly as I can find
from the literature, the proportion of apoptotic cells in those organs in
naive rats is pretty small.  Any other suggestions?

Thanks!

Martin Wessendorf



--
Patricia Masso-Welch, Ph.D.
Dept. Biotechnical and Clinical Laboratory Sciences
School of Medicine and Biomedical Sciences
University of Buffalo
3435 Main Street
Buffalo, NY  14214

Ph  716 829-5191
Fax [phone]
[hidden email]

[Gregg Sobocinski]

Do you have access to lactating rats? I've used post-lactation rat mammary glands. I believe the time period was 2-10 days post-lactation, but am not positive. Send me a personal message if you want me to look up the exact range for harvesting.

Regards,
~Gregg
 
Gregg Sobocinski
Microscope Imaging Specialist
University of Michigan, MCDB Dept.
Ann Arbor, Michigan
USA

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Martin Wessendorf
Sent: Friday, July 10, 2009 1:58 PM
To: [hidden email]
Subject: positive control for apoptosis

Dear all--

Can anyone suggest a good tissue to use as a positive control for
apoptosis?  We've considered liver and testes but as nearly as I can
find from the literature, the proportion of apoptotic cells in those
organs in naive rats is pretty small.  Any other suggestions?

Thanks!

Martin Wessendorf
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]

[Kathleen L Notarfrancesco]

We routinely use 2 - 4 day post-lactation mammary glands for our apoptosis positive controls.

  Kathy Notarfrancesco

----- Original Message -----
From: "Martin Wessendorf" <[hidden email]>
To: [hidden email]
Sent: Friday, July 10, 2009 1:58:29 PM GMT -05:00 US/Canada Eastern
Subject: positive control for apoptosis

Dear all--

Can anyone suggest a good tissue to use as a positive control for
apoptosis?  We've considered liver and testes but as nearly as I can
find from the literature, the proportion of apoptotic cells in those
organs in naive rats is pretty small.  Any other suggestions?

Thanks!

Martin Wessendorf
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]


--
Kathleen L Notarfrancesco
Abramson Family Cancer Research Institute
University of Pennsylvania
Department of Cancer Biology
627 BRB II/III   421 Curie Blvd.
Philadelphia, PA  19104-6160

Tel: [phone]       Fax: [phone]

[hidden email]

[anh2006]

Tonsil or spleen

On 07/10/09, Martin Wessendorf <[hidden email]> wrote:

Dear all--

Can anyone suggest a good tissue to use as a positive control for apoptosis?  We've considered liver and testes but as nearly as I can find from the literature, the proportion of apoptotic cells in those organs in naive rats is pretty small.  Any other suggestions?

Thanks!

Martin Wessendorf
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]

[Caroline Miller]

If you are doing tunel you can make your own by treating the sections  
with DNase prior to staining......otherwise I might try to find a  
decent tumour sample, with a little necrosis.

Happy Friday!
Caroline


Caroline Miller M.Sc DIC AIBMS
Co-manager
Gladstone Microscopy and Histology Core
J David Gladstone institute
1650 Owens Street
San Francisco
CA
94158

www.gladstone.ucsf.edu

On Jul 10, 2009, at 11:20, "Sobocinski, Gregg" <[hidden email]>  
wrote:

> Do you have access to lactating rats? I've used post-lactation rat  
> mammary glands. I believe the time period was 2-10 days post-
> lactation, but am not positive. Send me a personal message if you  
> want me to look up the exact range for harvesting.
>
> Regards,
> ~Gregg
>
> Gregg Sobocinski
> Microscope Imaging Specialist
> University of Michigan, MCDB Dept.
> Ann Arbor, Michigan
> USA
>
> -----Original Message-----
> From: Confocal Microscopy List  
> [mailto:[hidden email]] On Behalf Of Martin  
> Wessendorf
> Sent: Friday, July 10, 2009 1:58 PM
> To: [hidden email]
> Subject: positive control for apoptosis
>
> Dear all--
>
> Can anyone suggest a good tissue to use as a positive control for
> apoptosis?  We've considered liver and testes but as nearly as I can
> find from the literature, the proportion of apoptotic cells in those
> organs in naive rats is pretty small.  Any other suggestions?
>
> Thanks!
>
> Martin Wessendorf
> --
> Martin Wessendorf, Ph.D.                   office: [phone]
> Assoc Prof, Dept Neuroscience                 lab: [phone]
> University of Minnesota             Preferred FAX: [phone]
> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
> Minneapolis, MN  55455                    e-mail: [hidden email]