price for adding 405nm laser in Leica SP5

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Hi Everyone,

Right now we are planning to add 405nm laser line to our Leica SP5 II confocal system. I got a quote from the Leica rep but would like to know if that is the best price. I wonder if anyone can share your experience/quote with me. Thanks.

Long

[Zdenek Svindrych]

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Hi Long,

Is it Coherent CUBE? You may ask Coherent  for a quote. You'll find its
probably way less than half of the Leica price. But From Leica you get all
the service and guarantee and minimum downtime.
For a microscopy facility I'd go for Leica solution.
If it's my own microscope I'd consider bypassing Leica. But remember that
you need additional optical fiber, laser coupling and probably some parts
inside the scanhead. And don't forget the laser control and changes in the
software settings. So maybe the official way is the only solution for you.
Replacing the laser is so much simpler than adding a new one...

Good luck,

zdenek svindrych



---------- Původní zpráva ----------
Od: [hidden email] <[hidden email]>
Komu: [hidden email]
Datum: 5. 3. 2014 21:16:11
Předmět: price for adding 405nm laser in Leica SP5

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Hi Everyone,

Right now we are planning to add 405nm laser line to our Leica SP5 II
confocal system. I got a quote from the Leica rep but would like to know if
that is the best price. I wonder if anyone can share your experience/quote
with me. Thanks.

Long"

[Gregg Sobocinski]

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I second everything that Zdenek Svindrych has said. This is more than just
a laser. Your learning curve for performing this work will be extensive.

Good luck,
~Gregg

*Gregg Sobocinski*
Microscope Imaging Specialist
University of Michigan, MCDB Dept.
Ann Arbor, Michigan



On Wed, Mar 5, 2014 at 4:27 PM, Zdenek Svindrych <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi Long,
>
> Is it Coherent CUBE? You may ask Coherent  for a quote. You'll find its
> probably way less than half of the Leica price. But From Leica you get all
> the service and guarantee and minimum downtime.
> For a microscopy facility I'd go for Leica solution.
> If it's my own microscope I'd consider bypassing Leica. But remember that
> you need additional optical fiber, laser coupling and probably some parts
> inside the scanhead. And don't forget the laser control and changes in the
> software settings. So maybe the official way is the only solution for you.
> Replacing the laser is so much simpler than adding a new one...
>
> Good luck,
>
> zdenek svindrych
>
>
>
> ---------- Původní zpráva ----------
> Od: [hidden email] <[hidden email]>
> Komu: [hidden email]
> Datum: 5. 3. 2014 21:16:11
> Předmět: price for adding 405nm laser in Leica SP5
>
> "*****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi Everyone,
>
> Right now we are planning to add 405nm laser line to our Leica SP5 II
> confocal system. I got a quote from the Leica rep but would like to know if
> that is the best price. I wonder if anyone can share your experience/quote
> with me. Thanks.
>
> Long"
>

[Stephen Firth (Med)]

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Hi,
AFAIK this is not trivial, the 405nm comes in through a separate port, has
it's own AOTF, needs corrected optics - Lambda Blue objectives, there is
also correction in the scan head on the SP5, don't know whether this
correction is standard or needs to be added.
Cheers,
Steve



On 6 March 2014 06:44, [hidden email] <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi Everyone,
>
> Right now we are planning to add 405nm laser line to our Leica SP5 II
> confocal system. I got a quote from the Leica rep but would like to know if
> that is the best price. I wonder if anyone can share your experience/quote
> with me. Thanks.
>
> Long
>

--
Stephen Firth
Manager of Advanced Optical Microscopy
Monash Micro Imaging
Monash University

[Aryeh Weiss]

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On 3/6/14, 12:33 AM, Stephen Firth wrote:
>
> Hi,
> AFAIK this is not trivial, the 405nm comes in through a separate port, has
> it's own AOTF, needs corrected optics - Lambda Blue objectives, there is
> also correction in the scan head on the SP5, don't know whether this
> correction is standard or needs to be added.
> Cheers,
> Steve
>

You are correct. To add 405nm you will need a separate AOTF, and there
is a significant addition to the scanhead (combiner optics and
correction optics). It requires much more than just the laser.

--aryeh

>
> On 6 March 2014 06:44, [hidden email] <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Hi Everyone,
>>
>> Right now we are planning to add 405nm laser line to our Leica SP5 II
>> confocal system. I got a quote from the Leica rep but would like to know if
>> that is the best price. I wonder if anyone can share your experience/quote
>> with me. Thanks.
>>
>> Long
>>
>
>

--
Aryeh Weiss
Faculty of Engineering
Bar Ilan University
Ramat Gan 52900 Israel

Ph:  972-3-5317638
FAX: [phone]

[Craig Brideau]

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If you have a direct diode controlled 405 you can just modulate the laser
current.  Some people say this shortens the life of the laser but I have a
number of units rigged to operate this way and have had no problems.  This
way you don't need an AOTF at all.

Craig



On Wed, Mar 5, 2014 at 11:52 PM, Aryeh Weiss <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> On 3/6/14, 12:33 AM, Stephen Firth wrote:
>
>>
>> Hi,
>> AFAIK this is not trivial, the 405nm comes in through a separate port, has
>> it's own AOTF, needs corrected optics - Lambda Blue objectives, there is
>> also correction in the scan head on the SP5, don't know whether this
>> correction is standard or needs to be added.
>> Cheers,
>> Steve
>>
>>
> You are correct. To add 405nm you will need a separate AOTF, and there is
> a significant addition to the scanhead (combiner optics and correction
> optics). It requires much more than just the laser.
>
> --aryeh
>
>
>
>
>> On 6 March 2014 06:44, [hidden email] <[hidden email]> wrote:
>>
>>  *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Hi Everyone,
>>>
>>> Right now we are planning to add 405nm laser line to our Leica SP5 II
>>> confocal system. I got a quote from the Leica rep but would like to know
>>> if
>>> that is the best price. I wonder if anyone can share your
>>> experience/quote
>>> with me. Thanks.
>>>
>>> Long
>>>
>>>
>>
>>
>
> --
> Aryeh Weiss
> Faculty of Engineering
> Bar Ilan University
> Ramat Gan 52900 Israel
>
> Ph:  972-3-5317638
> FAX: [phone]
>

[Ben Abrams]

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Hi,

We added a 405 to our SP5.  You have to add correction lenses to the scanner
for your different objectives.  It is most cost effective to do all your
objectives at the time of installation.  We ended up not getting one for our
20x/0.7 and regret it.

Good luck,
Ben

[Arvydas Matiukas]

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Dear Core Managers,
 
Please share details if recently upgraded your LSM510 software
from AIM  to ZEN.
 
Our LSM510 is still in good condition but the computer which
is running AIM v4 software on Windows 2000 is 10 years old
and will not last much longer. Several years ago new Win7 computer
with upgrade to ZEN was $9-10k, and now should be cheaper
but our local rep does not know how much.
 
 
Best wishes,
Arvydas
~~~~~~~~~~~~~~~~~~~
Arvydas Matiukas, Ph.D.
Director of Confocal&Two-Photon Core
Department of Neurosci& Physiology
SUNY Upstate Medical University
766 Irving Ave., WH 3167
Syracuse, NY 13210

[Josef Gotzmann]

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dear Aryvdas,

the total price for upgrading is calculated on the number of upgrades you
need to pay for. in general i can also tell you that you can only upgrade to
ZEN2009 - later versions won't work with the 510s.
In addition the ZEN on 510machines has troubles with the SCSI interface.
whenever you start the computer Win7 will create an error message (I guess a
driver issue) and you won't have control of the lasers - to get started you
need to restart the software.

best,
Josef

On Fri, 7 Mar 2014 15:48:50 -0500, Arvydas Matiukas <[hidden email]>
wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Dear Core Managers,
>
>Please share details if recently upgraded your LSM510 software
>from AIM  to ZEN.
>
>Our LSM510 is still in good condition but the computer which
>is running AIM v4 software on Windows 2000 is 10 years old
>and will not last much longer. Several years ago new Win7 computer
>with upgrade to ZEN was $9-10k, and now should be cheaper
>but our local rep does not know how much.
>
>
>Best wishes,
>Arvydas
>~~~~~~~~~~~~~~~~~~~
>Arvydas Matiukas, Ph.D.
>Director of Confocal&Two-Photon Core
>Department of Neurosci& Physiology
>SUNY Upstate Medical University
>766 Irving Ave., WH 3167
>Syracuse, NY 13210