quencher far red

[Kevin Braeckmans]

Dear all,

 

Does someone know of a good quencher for dyes which emit in the far red region, such as e.g. Cy5? Tripan Blue does not seem to work for such far-red fluorophores, at least not in our hands.

 

Thanks,

 

Kevin

 

 

 

 

Prof. Dr. Kevin Braeckmans

Lab. General Biochemistry and Physical Pharmacy

Ghent University

Harelbekestraat 72

9000 Ghent

Belgium

Tel: [phone]

Fax: [phone]

 

 

[Kilgore, Jason]

 

** VENDOR RESPONSE **

 

Hi, Kevin,

 

QSY21 is a good choice.  See the following links for more information.

 

http://probes.invitrogen.com/handbook/tables/1569.html

 

http://probes.invitrogen.com/handbook/figures/1333.html

 

This is available in a succinimidyl ester form from Molecular Probes, product Q20132.

 

Cheers,

 

Jason

 

Jason A. Kilgore

Invitrogen Corporation

Molecular Probes Labeling and Detection Technologies

Technical Service Scientist

Eugene, OR  97402-9132

phone: [phone]

fax:      [phone]

[hidden email]

Please visit our website:  www.probes.invitrogen.com

 

 P Save trees.  Please don't print this e-mail unless you really need to.

---

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Kevin Braeckmans
Sent: Wednesday, November 12, 2008 10:19 AM
To: [hidden email]
Subject: quencher far red

Dear all,

 

Does someone know of a good quencher for dyes which emit in the far red region, such as e.g. Cy5? Tripan Blue does not seem to work for such far-red fluorophores, at least not in our hands.

 

Thanks,

 

Kevin

 

 

 

 

Prof. Dr. Kevin Braeckmans

Lab. General Biochemistry and Physical Pharmacy

Ghent University

Harelbekestraat 72

9000 Ghent

Belgium

Tel: [phone]

Fax: [phone]