Hi Arne,
I used a 3% hydrogen peroxide solution in PBS for 20 min at room
temperature to bleach GFP. I lost all the GFP signal and had no problem
with the following immunofluorescence. Maybe it will work also for the
mitochondria.
Good luck
Gabriel Lapointe
Arne Christensen wrote:
> Dear Listeners,
>
> I have encountered some very frustrating autofluorescence within salmon gill
> tissue. The autofluorescence is restricted to chloride (also called
> mitochondria-rich) cells. These cells are, not surprisingly, rich in
> mitochondria. Because components of mitochondria are known to
> autofluoresce, this may well be the source of my troubles.
>
> Is anybody aware of a good technique for quenching autofluorescence in
> general, or mitochondrial fluorescence specifically? The tissue must retain
> immunoreactivity. This tissue has been fixed two hours at RT in 4% PFA, and
> embedded in Tissue Tek OCT media.
>
> Thanks,
> Arne
>
>
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