Hi Stephen,
Thanks for the reply. Yes, the readout position of the z motor was not
what it is supposed to be and the image of the sample drifted obviously
while z was moving.
Best,
Yihan
Stephen Cody wrote:
> Dear Yihan,
>
> How are you observing this focus drift? Is the reported position of
> the z-drive from the optical encoder in the TE2000-E problematic? Or
> are you observing an apparently larger step judging by the image in
> the microscope / computer screen?
>
> Cheers
>
> Stephen Cody
>
> 2009/2/17 Yihan Lin <
[hidden email] <mailto:
[hidden email]>>
>
> Hello Everyone,
>
> I am building autofocus on the Nikon TE2000-E and having some
> problem controlling its z drive. I use serial port with the
> commands in the sdk to communicate with the microscope (through
> Labview). Whenever i sent a single-step (50nm) movement command to
> the motor (for example, SUC 1), 50% of the time it moved more than
> one step, sometimes even as large as 6 steps. It doesn't seem to
> be a closed-loop operation. Although I could correct for the
> movement with multiple commands, the imaging plane was drifting.
> Has anyone else encountered such a problem? I am not sure how
> does PFS work with such a motor.
>
> Thanks in advance!
>
> Yihan
>
>
>
>
> --
> Stephen H. Cody
> Consulting Microscopist
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