"Out of Office autoreply" courtesy

[Martin Wessendorf-2]

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By now, I expect everyone has deduced that it's a bad idea to be
subscribed to a list like this after you've set up an
"out-of-office-autoreply".  Please, if you have any doubts, unsubscribe
from the list till you return.  The link at the top of each posting will
allow you to do that.

Thanks!

Martin Wessendorf
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]

[lechristophe]

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Could you un_sub_scribe Mr Adams and ask him to sub_scribe again when he
returns?

On Fri, Oct 21, 2011 at 18:40, Martin Wessendorf <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
> *****
>
> By now, I expect everyone has deduced that it's a bad idea to be subscribed
> to a list like this after you've set up an "out-of-office-autoreply".
>  Please, if you have any doubts, unsubscribe from the list till you return.
>  The link at the top of each posting will allow you to do that.
>
> Thanks!
>
> Martin Wessendorf
> --
> Martin Wessendorf, Ph.D.                   office: [phone]
> Assoc Prof, Dept Neuroscience                 lab: [phone]
> University of Minnesota             Preferred FAX: [phone]
> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
> Minneapolis, MN  55455                    e-mail: [hidden email]
>

[rjpalmer]

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Yep.  And for the occasions that this doesn't happen and the auto-
replies become really intolerable, couldn't the moderators simply  
remove the address and request the person to sign up upon return?  No  
offense meant to Ben - this happens all the time.....

On Oct 21, 2011, at 12:40 PM, Martin Wessendorf wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> By now, I expect everyone has deduced that it's a bad idea to be
> subscribed to a list like this after you've set up an
> "out-of-office-autoreply".  Please, if you have any doubts,  
> unsubscribe
> from the list till you return.  The link at the top of each posting  
> will
> allow you to do that.
>
> Thanks!
>
> Martin Wessendorf
> --
> Martin Wessendorf, Ph.D.                   office: [phone]
> Assoc Prof, Dept Neuroscience                 lab: [phone]
> University of Minnesota             Preferred FAX: [phone]
> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
> Minneapolis, MN  55455                    e-mail: [hidden email]

Robert J. Palmer Jr., Ph.D.
Natl Inst Dental Craniofacial Res - Natl Insts Health
Oral Infection and Immunity Branch
Bldg 30, Room 310
30 Convent Drive
Bethesda MD 20892
ph 301-594-0025
fax [phone]

[MJ Tomaszewski]

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I believe it's outlook that has a 'only send one out of office reply to each
sender' option.

At least Ben will be back today. We should all ask him how his course went.

On Fri, Oct 21, 2011 at 12:50 PM, rjpalmer <[hidden email]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
> *****
>
> Yep.  And for the occasions that this doesn't happen and the auto-replies
> become really intolerable, couldn't the moderators simply remove the address
> and request the person to sign up upon return?  No offense meant to Ben -
> this happens all the time.....
>
> On Oct 21, 2011, at 12:40 PM, Martin Wessendorf wrote:
>
>  *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
>> *****
>>
>> By now, I expect everyone has deduced that it's a bad idea to be
>> subscribed to a list like this after you've set up an
>> "out-of-office-autoreply".  Please, if you have any doubts, unsubscribe
>> from the list till you return.  The link at the top of each posting will
>> allow you to do that.
>>
>> Thanks!
>>
>> Martin Wessendorf
>> --
>> Martin Wessendorf, Ph.D.                   office: [phone]
>> Assoc Prof, Dept Neuroscience                 lab: [phone]
>> University of Minnesota             Preferred FAX: [phone]
>> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
>> Minneapolis, MN  55455                    e-mail: [hidden email]
>>
>
> Robert J. Palmer Jr., Ph.D.
> Natl Inst Dental Craniofacial Res - Natl Insts Health
> Oral Infection and Immunity Branch
> Bldg 30, Room 310
> 30 Convent Drive
> Bethesda MD 20892
> ph 301-594-0025
> fax [phone]
>

[Unruh, Jay]

*****
To join, leave or search the confocal microscopy listserv, go to:
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*****

The ImageJ listserv requires a web based confirmation to submit to the list.  It can be a bit annoying, but it would eliminate issues like this.

Jay

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of MJ Tomaszewski
Sent: Friday, October 21, 2011 11:53 AM
To: [hidden email]
Subject: Re: "Out of Office autoreply" courtesy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I believe it's outlook that has a 'only send one out of office reply to each sender' option.

At least Ben will be back today. We should all ask him how his course went.

On Fri, Oct 21, 2011 at 12:50 PM, rjpalmer <[hidden email]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.u
> mn.edu/cgi-bin/wa?A0=confocalmicroscopy>
> *****
>
> Yep.  And for the occasions that this doesn't happen and the
> auto-replies become really intolerable, couldn't the moderators simply
> remove the address and request the person to sign up upon return?  No
> offense meant to Ben - this happens all the time.....
>
> On Oct 21, 2011, at 12:40 PM, Martin Wessendorf wrote:
>
>  *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.
>> umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
>> *****
>>
>> By now, I expect everyone has deduced that it's a bad idea to be
>> subscribed to a list like this after you've set up an
>> "out-of-office-autoreply".  Please, if you have any doubts,
>> unsubscribe from the list till you return.  The link at the top of
>> each posting will allow you to do that.
>>
>> Thanks!
>>
>> Martin Wessendorf
>> --
>> Martin Wessendorf, Ph.D.                   office: [phone]
>> Assoc Prof, Dept Neuroscience                 lab: [phone]
>> University of Minnesota             Preferred FAX: [phone]
>> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
>> Minneapolis, MN  55455                    e-mail: [hidden email]
>>
>
> Robert J. Palmer Jr., Ph.D.
> Natl Inst Dental Craniofacial Res - Natl Insts Health Oral Infection
> and Immunity Branch Bldg 30, Room 310
> 30 Convent Drive
> Bethesda MD 20892
> ph 301-594-0025
> fax [phone]
>

[Romin, Yevgeniy/Sloan Kettering Institute]

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To join, leave or search the confocal microscopy listserv, go to:
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*****

I don't know, I'm kind of getting used to seeing his name come up after each post, it would feel like something is missing if the moderators unsubscribed him :)

---------------------------------------------------
Yevgeniy Romin

Digital Microscopist
Memorial Sloan-Kettering Cancer Center
Molecular Cytology Core Facility
1275 York Ave. Box 333
New York, NY 10065
Tel.[phone]
Fax. [phone]
---------------------------------------------------

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Christophe Leterrier
Sent: Friday, October 21, 2011 12:48 PM
To: [hidden email]
Subject: Re: "Out of Office autoreply" courtesy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Could you un_sub_scribe Mr Adams and ask him to sub_scribe again when he
returns?

On Fri, Oct 21, 2011 at 18:40, Martin Wessendorf <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
> *****
>
> By now, I expect everyone has deduced that it's a bad idea to be subscribed
> to a list like this after you've set up an "out-of-office-autoreply".
>  Please, if you have any doubts, unsubscribe from the list till you return.
>  The link at the top of each posting will allow you to do that.
>
> Thanks!
>
> Martin Wessendorf
> --
> Martin Wessendorf, Ph.D.                   office: [phone]
> Assoc Prof, Dept Neuroscience                 lab: [phone]
> University of Minnesota             Preferred FAX: [phone]
> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
> Minneapolis, MN  55455                    e-mail: [hidden email]
>

 
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     Please note that this e-mail and any files transmitted with it may be
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     message to the intended recipient, you are hereby notified that any
     reading, dissemination, distribution, copying, or other use of this
     communication or any of its attachments is strictly prohibited.  If
     you have received this communication in error, please notify the
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     computer.

[Armstrong, Brian]

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To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

All 2000 plus members of the list should send him a "Welcome Back" e-mail when he returns.

Cheers,

Brian Armstrong PhD
Assistant Research Professor
Light Microscopy Core
Beckman Research Institute
City of Hope
1500 East Duarte Road
Duarte, CA 91010
626-256-4673 x62872

Light Microscopy Core Facility

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Yevgeniy Romin
Sent: Friday, October 21, 2011 10:00 AM
To: [hidden email]
Subject: Re: "Out of Office autoreply" courtesy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I don't know, I'm kind of getting used to seeing his name come up after each post, it would feel like something is missing if the moderators unsubscribed him :)

---------------------------------------------------
Yevgeniy Romin

Digital Microscopist
Memorial Sloan-Kettering Cancer Center
Molecular Cytology Core Facility
1275 York Ave. Box 333
New York, NY 10065
Tel.[phone]
Fax. [phone]
---------------------------------------------------

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Christophe Leterrier
Sent: Friday, October 21, 2011 12:48 PM
To: [hidden email]
Subject: Re: "Out of Office autoreply" courtesy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Could you un_sub_scribe Mr Adams and ask him to sub_scribe again when he
returns?

On Fri, Oct 21, 2011 at 18:40, Martin Wessendorf <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
> *****
>
> By now, I expect everyone has deduced that it's a bad idea to be subscribed
> to a list like this after you've set up an "out-of-office-autoreply".
>  Please, if you have any doubts, unsubscribe from the list till you return.
>  The link at the top of each posting will allow you to do that.
>
> Thanks!
>
> Martin Wessendorf
> --
> Martin Wessendorf, Ph.D.                   office: [phone]
> Assoc Prof, Dept Neuroscience                 lab: [phone]
> University of Minnesota             Preferred FAX: [phone]
> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
> Minneapolis, MN  55455                    e-mail: [hidden email]
>

 
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[Philippe clemenceau]

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My email browser is looking forward to his return !!

Philippe Clémenceau, Division Manager

Axiom Optics
1 Broadway, 14th floor
Cambridge,  MA 02142
www.axiomoptics.com
Ph:[phone]

Adaptive Optics for microscopy
Fluorescence Lifetime Imaging
EMCCDs cameras


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Armstrong, Brian
Sent: Friday, October 21, 2011 1:05 PM
To: [hidden email]
Subject: Re: "Out of Office autoreply" courtesy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

All 2000 plus members of the list should send him a "Welcome Back" e-mail
when he returns.

Cheers,

Brian Armstrong PhD
Assistant Research Professor
Light Microscopy Core
Beckman Research Institute
City of Hope
1500 East Duarte Road
Duarte, CA 91010
626-256-4673 x62872

Light Microscopy Core Facility

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Yevgeniy Romin
Sent: Friday, October 21, 2011 10:00 AM
To: [hidden email]
Subject: Re: "Out of Office autoreply" courtesy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I don't know, I'm kind of getting used to seeing his name come up after each
post, it would feel like something is missing if the moderators unsubscribed
him :)

---------------------------------------------------
Yevgeniy Romin

Digital Microscopist
Memorial Sloan-Kettering Cancer Center
Molecular Cytology Core Facility
1275 York Ave. Box 333
New York, NY 10065
Tel.[phone]
Fax. [phone]
---------------------------------------------------

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Christophe Leterrier
Sent: Friday, October 21, 2011 12:48 PM
To: [hidden email]
Subject: Re: "Out of Office autoreply" courtesy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Could you un_sub_scribe Mr Adams and ask him to sub_scribe again when he
returns?

On Fri, Oct 21, 2011 at 18:40, Martin Wessendorf <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu
/cgi-bin/wa?A0=confocalmicroscopy>
> *****
>
> By now, I expect everyone has deduced that it's a bad idea to be
subscribed
> to a list like this after you've set up an "out-of-office-autoreply".
>  Please, if you have any doubts, unsubscribe from the list till you
return.

>  The link at the top of each posting will allow you to do that.
>
> Thanks!
>
> Martin Wessendorf
> --
> Martin Wessendorf, Ph.D.                   office: [phone]
> Assoc Prof, Dept Neuroscience                 lab: [phone]
> University of Minnesota             Preferred FAX: [phone]
> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
> Minneapolis, MN  55455                    e-mail: [hidden email]
>

 
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     message to the intended recipient, you are hereby notified that any
     reading, dissemination, distribution, copying, or other use of this
     communication or any of its attachments is strictly prohibited.  If
     you have received this communication in error, please notify the
     sender immediately by replying to this message and deleting this
     message, any attachments, and all copies and backups from your
     computer.


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financial information). Because this e-mail has been sent without
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[Martin Wessendorf-2]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

On 10/21/2011 11:56 AM, Unruh, Jay wrote:

> The ImageJ listserv requires a web based confirmation to submit to the list.  It can be a bit annoying, but it would eliminate issues like this.

Good idea--this has now been done.  If confirming each posting ends up
being too annoying to the group, let me know.  If there's a consensus,
we can turn it back off again.

Martin Wessendorf
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]

[Zoltan]

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To join, leave or search the confocal microscopy listserv, go to:
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Hear, hear!

On Fri, Oct 21, 2011 at 7:04 PM, Armstrong, Brian <[hidden email]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> All 2000 plus members of the list should send him a "Welcome Back" e-mail
> when he returns.
>
> Cheers,
>
> Brian Armstrong PhD
> Assistant Research Professor
> Light Microscopy Core
> Beckman Research Institute
> City of Hope
> 1500 East Duarte Road
> Duarte, CA 91010
> 626-256-4673 x62872
>
> Light Microscopy Core Facility
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Yevgeniy Romin
> Sent: Friday, October 21, 2011 10:00 AM
> To: [hidden email]
> Subject: Re: "Out of Office autoreply" courtesy
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> I don't know, I'm kind of getting used to seeing his name come up after
> each post, it would feel like something is missing if the moderators
> unsubscribed him :)
>
> ---------------------------------------------------
> Yevgeniy Romin
>
> Digital Microscopist
> Memorial Sloan-Kettering Cancer Center
> Molecular Cytology Core Facility
> 1275 York Ave. Box 333
> New York, NY 10065
> Tel.[phone]
> Fax. [phone]
> ---------------------------------------------------
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Christophe Leterrier
> Sent: Friday, October 21, 2011 12:48 PM
> To: [hidden email]
> Subject: Re: "Out of Office autoreply" courtesy
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Could you un_sub_scribe Mr Adams and ask him to sub_scribe again when he
> returns?
>
> On Fri, Oct 21, 2011 at 18:40, Martin Wessendorf <[hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
> > *****
> >
> > By now, I expect everyone has deduced that it's a bad idea to be
> subscribed
> > to a list like this after you've set up an "out-of-office-autoreply".
> >  Please, if you have any doubts, unsubscribe from the list till you
> return.
> >  The link at the top of each posting will allow you to do that.
> >
> > Thanks!
> >
> > Martin Wessendorf
> > --
> > Martin Wessendorf, Ph.D.                   office: [phone]
> > Assoc Prof, Dept Neuroscience                 lab: [phone]
> > University of Minnesota             Preferred FAX: [phone]
> > 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
> > Minneapolis, MN  55455                    e-mail: [hidden email]
> >
>
>
>
>     =====================================================================
>
>     Please note that this e-mail and any files transmitted with it may be
>     privileged, confidential, and protected from disclosure under
>     applicable law. If the reader of this message is not the intended
>     recipient, or an employee or agent responsible for delivering this
>     message to the intended recipient, you are hereby notified that any
>     reading, dissemination, distribution, copying, or other use of this
>     communication or any of its attachments is strictly prohibited.  If
>     you have received this communication in error, please notify the
>     sender immediately by replying to this message and deleting this
>     message, any attachments, and all copies and backups from your
>     computer.
>
>
> ---------------------------------------------------------------------
> *SECURITY/CONFIDENTIALITY WARNING:
> This message and any attachments are intended solely for the individual or
> entity to which they are addressed. This communication may contain
> information that is privileged, confidential, or exempt from disclosure
> under applicable law (e.g., personal health information, research data,
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> encryption, individuals other than the intended recipient may be able to
> view the information, forward it to others or tamper with the information
> without the knowledge or consent of the sender. If you are not the intended
> recipient, or the employee or person responsible for delivering the message
> to the intended recipient, any dissemination, distribution or copying of the
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Zoltan Cseresnyes

--------------------------------------------
Q: Why is this email four sentences or less?
A: http://four.sentenc.es

[Ben Abrams]

Well, if I could shrink down to <~200nm I would, then most of you would not be able to see me.  I obviously did not realize that my auto reply would post directly to the list.  I am extremely embarrassed about this.   I hope everyone will accept my deep and sincere apology.

[Peter Werner]

*****
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And I hate to point this out, but the question I originally asked
(concerning the definition of Airy Units) has been drowned out by all the
commentary about the "Out of Office autoreply" that my initial email generated.

If nobody has an answer to the question, no worries, but I'd hate to see the
topic get lost under the weight of discussion of listserv function.

[Martin Wessendorf-2]

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On 10/21/2011 11:56 AM, Unruh, Jay wrote:

> The ImageJ listserv requires a web based confirmation to submit to the list.  It can be a bit annoying, but it would eliminate issues like this.

Good idea--this has now been done.  If confirming each posting ends up
being too annoying to the group, let me know.  If there's a consensus,
we can turn it back off again.

Martin Wessendorf
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                     email: [hidden email]

[Zoltan]

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I think the confirmation request is a good idea, a very minor inconvenience
compared to the annoyance it successfully eliminates.

Zoltan

On Sun, Oct 23, 2011 at 10:50 PM, Martin Wessendorf <[hidden email]> wrote:

> The ImageJ listserv requires a web based confirmation to submit to the
>> list.  It can be a bit annoying, but it would eliminate issues like this.
>>
>
> Good idea--this has now been done.  If confirming each posting ends up
> being too annoying to the group, let me know.  If there's a consensus, we
> can turn it back off again.
>
>
> Martin Wessendorf
>

--

Zoltan Cseresnyes

--------------------------------------------
Q: Why is this email four sentences or less?
A: http://four.sentenc.es

[Adrian Smith-6]

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On 22/10/2011, at 3:40 AM, Martin Wessendorf wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> By now, I expect everyone has deduced that it's a bad idea to be subscribed to a list like this after you've set up an "out-of-office-autoreply".  Please, if you have any doubts, unsubscribe from the list till you return.  The link at the top of each posting will allow you to do that.
>
> Thanks!
>
> Martin Wessendorf

Actually it should "just work".

If the list server is correctly configured and the email server sending the auto-responder is correctly configured then no auto-replies should go to the list.

So the people who need to be take a look here are the list-admin (is the list setting the correct headers) and the sys admins at UCSF (have they configured their email server auto responder settings to correctly ignore list posts).

Given the low frequency with which this occurs (in terms of individuals - not in terms of the number of emails!) on this list compared to others I'm on (cytometry mailing list) I suspect the problem may be at the UCSF end.

(Alternatively, maybe Ben left a auto responder running on his email client and left his computer running…?)

Adrian

[Guy Cox-2]

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You are right, it has been totally drowned out!

The Airy unit is defined by the size of the Airy disk, that is the size
of the disk representing the image of a point object.  Airy was an
astronomer and thus derived it by reference to stars (which, though
huge, are so far away that they appear as point objects).  John Strutt,
Lord Rayleigh, proposed a general resolution criterion that two objects
can be considered resolved if the maximum of one Airy disk lies on the
first minimum of the other.  This criterion, the radius of the central
disk (ignoring surrounding haloes) is given by  r   =  0.61 lambda  /
NA, where lambda is the wavelength of the light being used.  

                                         Guy


Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
     http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
Madsen Building F09, University of Sydney, NSW 2006

Phone [phone]     Fax [phone]
             Mobile [phone]
______________________________________________
      http://www.guycox.net
 


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Peter G. Werner
Sent: Sunday, 23 October 2011 4:55 AM
To: [hidden email]
Subject: Re: "Out of Office autoreply" courtesy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

And I hate to point this out, but the question I originally asked
(concerning the definition of Airy Units) has been drowned out by all
the
commentary about the "Out of Office autoreply" that my initial email
generated.

If nobody has an answer to the question, no worries, but I'd hate to see
the
topic get lost under the weight of discussion of listserv function.

-----
No virus found in this message.
Checked by AVG - www.avg.com
Version: 10.0.1411 / Virus Database: 1522/3970 - Release Date: 10/23/11

[James Mansfield]

*****
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Hi,

There are several good online descriptions of airy disks and image resolution. Two good ones are:

http://www.microscopyu.com/tutorials/java/imageformation/airyna/

http://www.olympusmicro.com/primer/java/microscopy/airydiscs2/

Jim


James R. Mansfield
Director, Tissue Analysis Applications
Caliper Life Sciences, Inc.
68 Elm Street, Hopkinton, MA 01748
Office: [phone]
Cell: [phone]
Email: [hidden email]
www.caliperls.com
www.cri-inc.com

Need to send me large files? Use my YouSendIt DropBox:
https://dropbox.yousendit.com/Mansfield-Dropbox


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Guy Cox
Sent: Wednesday, October 26, 2011 5:46 AM
To: [hidden email]
Subject: Airy Units - was: RE: "Out of Office autoreply" courtesy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

You are right, it has been totally drowned out!

The Airy unit is defined by the size of the Airy disk, that is the size
of the disk representing the image of a point object.  Airy was an
astronomer and thus derived it by reference to stars (which, though
huge, are so far away that they appear as point objects).  John Strutt,
Lord Rayleigh, proposed a general resolution criterion that two objects
can be considered resolved if the maximum of one Airy disk lies on the
first minimum of the other.  This criterion, the radius of the central
disk (ignoring surrounding haloes) is given by  r   =  0.61 lambda  /
NA, where lambda is the wavelength of the light being used.

                                         Guy


Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
     http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
Madsen Building F09, University of Sydney, NSW 2006

Phone [phone]     Fax [phone]
             Mobile [phone]
______________________________________________
      http://www.guycox.net



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Peter G. Werner
Sent: Sunday, 23 October 2011 4:55 AM
To: [hidden email]
Subject: Re: "Out of Office autoreply" courtesy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

And I hate to point this out, but the question I originally asked
(concerning the definition of Airy Units) has been drowned out by all
the
commentary about the "Out of Office autoreply" that my initial email
generated.

If nobody has an answer to the question, no worries, but I'd hate to see
the
topic get lost under the weight of discussion of listserv function.

-----
No virus found in this message.
Checked by AVG - www.avg.com
Version: 10.0.1411 / Virus Database: 1522/3970 - Release Date: 10/23/11

============================================================= The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. =============================================================

====================================================================================

The information contained in this e-mail message is intended only
for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient
or an agent responsible for delivering it to the intended recipient,
you are hereby notified that you have received this document in
error and that any review, dissemination, distribution, or copying
of this message is strictly prohibited. If you have received this
communication in error, please notify us immediately by e-mail, and
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====================================================================================

[Armstrong, Brian]

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*****

Guy (and list), in a couple of super-resolution talks I've attended they were using 0.51 instead of 0.61 for the constant. Do you know the rationale behind this?
Thanks, Brian

Brian Armstrong PhD
Light Microscopy Core
Beckman Research Institute
1450 East Duarte Rd
Duarte, CA 91010
626-256-4673 x62872
http://www.cityofhope.org/SharedResources/LightMicroscopy/LightMicroHome.htm
 
 
-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Guy Cox
Sent: Wednesday, October 26, 2011 2:46 AM
To: [hidden email]
Subject: Airy Units - was: RE: "Out of Office autoreply" courtesy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

You are right, it has been totally drowned out!

The Airy unit is defined by the size of the Airy disk, that is the size
of the disk representing the image of a point object.  Airy was an
astronomer and thus derived it by reference to stars (which, though
huge, are so far away that they appear as point objects).  John Strutt,
Lord Rayleigh, proposed a general resolution criterion that two objects
can be considered resolved if the maximum of one Airy disk lies on the
first minimum of the other.  This criterion, the radius of the central
disk (ignoring surrounding haloes) is given by  r   =  0.61 lambda  /
NA, where lambda is the wavelength of the light being used.  

                                         Guy


Optical Imaging Techniques in Cell Biology
by Guy Cox    CRC Press / Taylor & Francis
     http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
Madsen Building F09, University of Sydney, NSW 2006

Phone [phone]     Fax [phone]
             Mobile [phone]
______________________________________________
      http://www.guycox.net
 


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Peter G. Werner
Sent: Sunday, 23 October 2011 4:55 AM
To: [hidden email]
Subject: Re: "Out of Office autoreply" courtesy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

And I hate to point this out, but the question I originally asked
(concerning the definition of Airy Units) has been drowned out by all
the
commentary about the "Out of Office autoreply" that my initial email
generated.

If nobody has an answer to the question, no worries, but I'd hate to see
the
topic get lost under the weight of discussion of listserv function.

-----
No virus found in this message.
Checked by AVG - www.avg.com
Version: 10.0.1411 / Virus Database: 1522/3970 - Release Date: 10/23/11


---------------------------------------------------------------------
*SECURITY/CONFIDENTIALITY WARNING:  
This message and any attachments are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender.

---------------------------------------------------------------------

[lechristophe]

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*****

I think the formula with 0.51 is the full width at half maximum (FWHM), i.e.
the distance between the two points where the intensity is half the maximum,
whereas the one with 0.61 is the radius of the Airy disk, i.e. the distance
between the maximum and the first minimum.

Christophe

On Wed, Oct 26, 2011 at 17:25, Armstrong, Brian <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Guy (and list), in a couple of super-resolution talks I've attended they
> were using 0.51 instead of 0.61 for the constant. Do you know the rationale
> behind this?
> Thanks, Brian
>
> Brian Armstrong PhD
> Light Microscopy Core
> Beckman Research Institute
> 1450 East Duarte Rd
> Duarte, CA 91010
> 626-256-4673 x62872
>
> http://www.cityofhope.org/SharedResources/LightMicroscopy/LightMicroHome.htm
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Guy Cox
> Sent: Wednesday, October 26, 2011 2:46 AM
> To: [hidden email]
> Subject: Airy Units - was: RE: "Out of Office autoreply" courtesy
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> You are right, it has been totally drowned out!
>
> The Airy unit is defined by the size of the Airy disk, that is the size
> of the disk representing the image of a point object.  Airy was an
> astronomer and thus derived it by reference to stars (which, though
> huge, are so far away that they appear as point objects).  John Strutt,
> Lord Rayleigh, proposed a general resolution criterion that two objects
> can be considered resolved if the maximum of one Airy disk lies on the
> first minimum of the other.  This criterion, the radius of the central
> disk (ignoring surrounding haloes) is given by  r   =  0.61 lambda  /
> NA, where lambda is the wavelength of the light being used.
>
>                                         Guy
>
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox    CRC Press / Taylor & Francis
>     http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Australian Centre for Microscopy & Microanalysis,
> Madsen Building F09, University of Sydney, NSW 2006
>
> Phone [phone]     Fax [phone]
>             Mobile [phone]
> ______________________________________________
>      http://www.guycox.net
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Peter G. Werner
> Sent: Sunday, 23 October 2011 4:55 AM
> To: [hidden email]
> Subject: Re: "Out of Office autoreply" courtesy
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> And I hate to point this out, but the question I originally asked
> (concerning the definition of Airy Units) has been drowned out by all
> the
> commentary about the "Out of Office autoreply" that my initial email
> generated.
>
> If nobody has an answer to the question, no worries, but I'd hate to see
> the
> topic get lost under the weight of discussion of listserv function.
>
> -----
> No virus found in this message.
> Checked by AVG - www.avg.com
> Version: 10.0.1411 / Virus Database: 1522/3970 - Release Date: 10/23/11
>
>
> ---------------------------------------------------------------------
> *SECURITY/CONFIDENTIALITY WARNING:
> This message and any attachments are intended solely for the individual or
> entity to which they are addressed. This communication may contain
> information that is privileged, confidential, or exempt from disclosure
> under applicable law (e.g., personal health information, research data,
> financial information). Because this e-mail has been sent without
> encryption, individuals other than the intended recipient may be able to
> view the information, forward it to others or tamper with the information
> without the knowledge or consent of the sender. If you are not the intended
> recipient, or the employee or person responsible for delivering the message
> to the intended recipient, any dissemination, distribution or copying of the
> communication is strictly prohibited. If you received the communication in
> error, please notify the sender immediately by replying to this message and
> deleting the message and any accompanying files from your system. If, due to
> the security risks, you do not wish to receive further communications via
> e-mail, please reply to this message and inform the sender that you do not
> wish to receive further e-mail from the sender.
>
> ---------------------------------------------------------------------
>

[James Pawley]

*****
To join, leave or search the confocal microscopy listserv, go to:
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*****

>*****
>To join, leave or search the confocal microscopy listserv, go to:
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>*****


Hi All,

The Rayleigh criterion described by Guy implies that exactly centered
between the two peaks (of equal brightness!) the intensity will drop
to 75% of that at the peaks (often referred to as 25% contrast). This
value seems to have been chosen because it was felt that the eye
could not reliably detect a smaller "darkening" and more to the
point, the maths were easier.

Enter electronic image capture and display, where by fiddling the
contrast and brightness one can "enhance" even a slight dip in
intensity enough to make it visible to the eye, leading to the
Sparrow Criterion, where the 0.51 comes from.

The point is that these neither of these criteria are really very
practically useful for a number of reasons:

1) Electronic images must be quantized in space and intensity. If you
use Nyquist and put 2 or 3 pixels between the peaks, then the
"darkest" one will be a lot brighter than 75% because the math
function describing the sum of the two Airy Disks shows only a very
narrow dip to 75% and this will be averaged with "the sides of the
canyon," if you see what I mean. So, depending on exactly how the
point object lines up on the sampling grid, at Rayleigh spacing, the
inter-peak contrast is closer to 10% than 25%.

2) Rayleigh wasn't worried about photons. We are, because of Poisson
Statistics. To see, say, 10% contrast with one-sigma reliability
requires at least 100 photons to be counted from the dimmest pixel.
In fluorescence imaging, this is only likely to occur on fixed
specimens.

3) Rayleigh was interested in bright objects on a black background.
This fits well with fluorescence microscopy but not so well with any
kind of brightfield because in the latter case the "background"
(average intensity) has considerable Poisson Noise superimposed upon
it and this can "create" apparent features out of random statistical
fluctuations. So you may need to collect many more photons to
discriminate real features reliably in brightfield. Black backgrounds
do not have this problem

4) All this nice math falls apart when the two point objects are not
of equal brightness. And they seldom are. Ditto, if there is
significant background.

But getting back to the original question: If "one Airy unit" is the
radius of the Airy disk to the first zero, and as it turns out, this
is very nearly equal to the full-width-at-half-maximum of the central
peak, should we use One Airy as the default pinhole setting or 2 Airy
which would include the whole peak? And how does this decision affect
the signal levels detected from features that are larger than points?

JP
***************************************************************************
Prof. James B. Pawley,                          Ph.
608-238-3953                        
21. N. Prospect Ave. Madison, WI 53726 USA
[hidden email]
3D Microscopy of Living Cells Course, June 10-22, 2012, UBC, Vancouver Canada
Info: http://www.3dcourse.ubc.ca/        Applications accepted after 11/15/12
               "If it ain't diffraction, it must be statistics." Anon.

>Guy (and list), in a couple of super-resolution talks I've attended
>they were using 0.51 instead of 0.61 for the constant. Do you know
>the rationale behind this?
>Thanks, Brian
>
>Brian Armstrong PhD
>Light Microscopy Core
>Beckman Research Institute
>1450 East Duarte Rd
>Duarte, CA 91010
>626-256-4673 x62872
>http://www.cityofhope.org/SharedResources/LightMicroscopy/LightMicroHome.htm
>
>
>-----Original Message-----
>From: Confocal Microscopy List
>[mailto:[hidden email]] On Behalf Of Guy Cox
>Sent: Wednesday, October 26, 2011 2:46 AM
>To: [hidden email]
>Subject: Airy Units - was: RE: "Out of Office autoreply" courtesy
>
>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>You are right, it has been totally drowned out!
>
>The Airy unit is defined by the size of the Airy disk, that is the size
>of the disk representing the image of a point object.  Airy was an
>astronomer and thus derived it by reference to stars (which, though
>huge, are so far away that they appear as point objects).  John Strutt,
>Lord Rayleigh, proposed a general resolution criterion that two objects
>can be considered resolved if the maximum of one Airy disk lies on the
>first minimum of the other.  This criterion, the radius of the central
>disk (ignoring surrounding haloes) is given by  r   =  0.61 lambda  /
>NA, where lambda is the wavelength of the light being used.
>
>                                          Guy
>
>
>Optical Imaging Techniques in Cell Biology
>by Guy Cox    CRC Press / Taylor & Francis
>      http://www.guycox.com/optical.htm
>______________________________________________
>Associate Professor Guy Cox, MA, DPhil(Oxon)
>Australian Centre for Microscopy & Microanalysis,
>Madsen Building F09, University of Sydney, NSW 2006
>
>Phone [phone]     Fax [phone]
>              Mobile [phone]
>______________________________________________
>       http://www.guycox.net
>
>
>
>-----Original Message-----
>From: Confocal Microscopy List [mailto:[hidden email]]
>On Behalf Of Peter G. Werner
>Sent: Sunday, 23 October 2011 4:55 AM
>To: [hidden email]
>Subject: Re: "Out of Office autoreply" courtesy
>
>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>And I hate to point this out, but the question I originally asked
>(concerning the definition of Airy Units) has been drowned out by all
>the
>commentary about the "Out of Office autoreply" that my initial email
>generated.
>
>If nobody has an answer to the question, no worries, but I'd hate to see
>the
>topic get lost under the weight of discussion of listserv function.
>
>-----
>No virus found in this message.
>Checked by AVG - www.avg.com
>Version: 10.0.1411 / Virus Database: 1522/3970 - Release Date: 10/23/11
>
>
>---------------------------------------------------------------------
>*SECURITY/CONFIDENTIALITY WARNING:
>This message and any attachments are intended solely for the
>individual or entity to which they are addressed. This communication
>may contain information that is privileged, confidential, or exempt
>from disclosure under applicable law (e.g., personal health
>information, research data, financial information). Because this
>e-mail has been sent without encryption, individuals other than the
>intended recipient may be able to view the information, forward it
>to others or tamper with the information without the knowledge or
>consent of the sender. If you are not the intended recipient, or the
>employee or person responsible for delivering the message to the
>intended recipient, any dissemination, distribution or copying of
>the communication is strictly prohibited. If you received the
>communication in error, please notify the sender immediately by
>replying to this message and deleting the message and any
>accompanying files from your system. If, due to the security risks,
>you do not wish to receive further communications via e-mail, please
>reply to this message and inform the sender that you do not wish to
>receive further e-mail from the sender.
>
>---------------------------------------------------------------------

--
***************************************************************************
Prof. James B. Pawley,                          Ph.
608-238-3953                        
21. N. Prospect Ave. Madison, WI 53726 USA
[hidden email]
3D Microscopy of Living Cells Course, June 10-22, 2012, UBC, Vancouver Canada
Info: http://www.3dcourse.ubc.ca/        Applications accepted after 11/15/12
               "If it ain't diffraction, it must be statistics." Anon.