reflected light optical sectioning
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Tim Holmes-2 |
reflected light optical sectioning
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Dear all: I want to scan an in-vitro tissue sample using broadband
(i.e., as white as possible – e.g., tungsten or halogen lamp) standard
koehler illumination, reflected light, with optical sectioning. There is no
confocal scanning to be done. I want to use a standard reflected light configuration (epi-illuminated), upright microscope. The
only special component is that it needs to have optical sectioning
capabilities, i.e., a stepper motor attached to the z-stage or a piezo device
attached to the objective and software to drive it (e.g., Media Cybernetics).
I have recently located to St. Louis, so I am looking for a facility/person within
driving distance who might have such a set up and who might be willing to help
me collect the image stacks. If you can give me a pointer to a facility/person
nearby please let me know. Nearby cities are St. Louis (obviously), Kansas
City, Columbia (Mo.), Chicago, Champaign. Thanks a lot. Tim Holmes Lickenbrock Technologies |
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This year's Nobel Prize for Chemistry has been awarded
to Osamu Shimomura, Martin Chalfie
and Roger Tsien for their work on discovering GFP and making it a
useful tool in cell biology. I would guess that many of us on this list
know one or more of these people personally, and all of us have carried out
research which they have made possible. It is a tremendous honour, but one
which is well deserved, and I think that all of us in this community would like
to join in offering our congratulations.
Guy Optical Imaging Techniques in Cell Biology From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Tim Holmes Sent: Wednesday, 8 October 2008 7:14 AM To: [hidden email] Subject: reflected light optical sectioning Dear
all: I want to scan an in-vitro tissue
sample using broadband (i.e., as white as possible – e.g., tungsten or halogen
lamp) standard koehler illumination, reflected light, with optical
sectioning. There is no confocal scanning to be done. I want to use
a standard reflected light configuration (epi-illuminated),
upright microscope. The only special component is that it needs to have
optical sectioning capabilities, i.e., a stepper motor attached to the z-stage
or a piezo device attached to the objective and software to drive it (e.g.,
Media Cybernetics). I have recently located to St. Louis, so I am looking
for a facility/person within driving distance who might have such a set up and
who might be willing to help me collect the image stacks. If you can give
me a pointer to a facility/person nearby please let me know. Nearby cities
are St. Louis (obviously), Kansas City, Columbia (Mo.), Chicago,
Champaign. Thanks a
lot. Tim
Holmes Lickenbrock
Technologies No virus found in this incoming message. No virus found in this outgoing message. |
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Periasamy, Ammasi (ap3t) |
Re: Nobel Prize for GFP
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I agree with Guy Cox. They deserve this award even though it took
some time to recognize their contribution. The investigation of biological system using imaging technology
tremendously improved because of their contribution. Congratulations!! Ammasi From: Confocal Microscopy
List [mailto:[hidden email]] On Behalf Of Guy Cox This
year's Nobel Prize for Chemistry has been awarded to Osamu Shimomura, Martin
Chalfie and Roger Tsien for their work on discovering GFP and making it a
useful tool in cell biology. I would guess that many of us on this list
know one or more of these people personally, and all of us have carried out
research which they have made possible. It is a tremendous honour, but
one which is well deserved, and I think that all of us in this community would
like to join in offering our congratulations.
Guy Ammasi Periasamy, Ph.D. Director, Keck Center for
Cellular Imaging (KCCI) Professor of Biology and
Biomedical Engineering Biology, Gilmer Hall (064),
McCormick Rd University of Virginia Charlottesville, VA 22904 Voice: 434-243-7602 (Office);
982-4869 (lab) Fax:434-982-5210; Email:[hidden email] http//:www.kcci.virginia.edu ************************ Workshop on FRET Microscopy,
March 3-7, 2009 http://www.kcci.virginia.edu/workshop/workshop2009/index.php ************************* |
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Jon Ekman-2 |
Re: reflected light optical sectioning
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In reply to this post by Tim Holmes-2
Up in Urbana/Champaign IL, at the Beckman Institute, we have an upright
Zeiss AxioImager A1 with an Optronics Microfire color CCD camera and motorized XY&Z
with reflected light capability. We use StereoInvestigator V8.21 software
to acquire Z-stacks on that system and have many options for software image
analysis afterwards. If you think we can help, contact me offline. Web: http:// itg.uiuc.edu Cheers, Jon Jonathan M. Ekman Imaging Technology Group Beckman Institute for Advanced Science and Technology University of Illinois at Urbana-Champaign 405 N. Matthews Avenue Urbana, IL 61801 USA Tel: 217-244-6292 Fax: 217-244-6219 From: Confocal Microscopy
List [mailto:[hidden email]] On Behalf Of Tim Holmes Dear
all: I
want to scan an in-vitro tissue sample using broadband (i.e., as white as
possible – e.g., tungsten or halogen lamp) standard koehler illumination,
reflected light, with optical sectioning. There is no confocal scanning
to be done. I want to use a standard reflected light configuration
(epi-illuminated), upright microscope. The only special component is that
it needs to have optical sectioning capabilities, i.e., a stepper motor
attached to the z-stage or a piezo device attached to the objective and
software to drive it (e.g., Media Cybernetics). I have recently located
to St. Louis, so I am looking for a facility/person within driving distance who
might have such a set up and who might be willing to help me collect the image
stacks. If you can give me a pointer to a facility/person nearby please
let me know. Nearby cities are St. Louis (obviously), Kansas City,
Columbia (Mo.), Chicago, Champaign. Thanks
a lot. Tim
Holmes Lickenbrock
Technologies |
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In reply to this post by Periasamy, Ammasi (ap3t)
Great news ! I also have a thought for Douglas Prasher, because he cloned it and first had the idea of making fusion proteins, but went out of business just after publishing the sequence because of a lack of grants.
http://www.conncoll.edu/ccacad/zimmer/GFP-ww/prasher.html Christophe On Wed, Oct 8, 2008 at 16:05, Periasamy, Ammasi (ap3t) <[hidden email]> wrote:
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In reply to this post by Periasamy, Ammasi (ap3t)
I took a course in college with Chalfie, and never knew of his lab's
involvement with GFP, something I now work with every day! Thanks for the heads-up, Guy. Periasamy, Ammasi (ap3t) wrote: > > I agree with Guy Cox. They deserve this award even though it took some > time to recognize their contribution. > > The investigation of biological system using imaging technology > tremendously improved because of their contribution. > > Congratulations!! > > > > Ammasi > > > > > > > > *From:* Confocal Microscopy List > [mailto:[hidden email]] *On Behalf Of *Guy Cox > *Sent:* Wednesday, October 08, 2008 8:22 AM > *To:* [hidden email] > *Subject:* Nobel Prize for GFP > > > > This year's Nobel Prize for Chemistry has been awarded to Osamu > Shimomura, Martin Chalfie and Roger Tsien for their work on > discovering GFP and making it a useful tool in cell biology. I would > guess that many of us on this list know one or more of these people > personally, and all of us have carried out research which they have > made possible. It is a tremendous honour, but one which is well > deserved, and I think that all of us in this community would like to > join in offering our congratulations. > > > > > Guy > > Ammasi Periasamy, Ph.D. > > Director, Keck Center for Cellular Imaging (KCCI) > > Professor of Biology and Biomedical Engineering > > Biology, Gilmer Hall (064), McCormick Rd > > University of Virginia > > Charlottesville, VA 22904 > > Voice: 434-243-7602 (Office); 982-4869 (lab) > > Fax:434-982-5210; Email:[hidden email] > > http//:www.kcci.virginia.edu > > ************************ > > Workshop on FRET Microscopy, March 3-7, 2009 > > http://www.kcci.virginia.edu/workshop/workshop2009/index.php > > ************************* > > > |
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GREAT
FIRST CLASS PRODIGY Il giorno 08/ott/08, alle ore 16:51, Peter Carroll ha scritto: > I took a course in college with Chalfie, and never knew of his lab's > involvement with GFP, something I now work with every day! Thanks > for the heads-up, Guy. > > Periasamy, Ammasi (ap3t) wrote: >> >> I agree with Guy Cox. They deserve this award even though it took >> some time to recognize their contribution. >> >> The investigation of biological system using imaging technology >> tremendously improved because of their contribution. >> >> Congratulations!! >> >> >> Ammasi >> >> >> >> >> *From:* Confocal Microscopy List [mailto:[hidden email] >> ] *On Behalf Of *Guy Cox >> *Sent:* Wednesday, October 08, 2008 8:22 AM >> *To:* [hidden email] >> *Subject:* Nobel Prize for GFP >> >> >> This year's Nobel Prize for Chemistry has been awarded to Osamu >> Shimomura, Martin Chalfie and Roger Tsien for their work on >> discovering GFP and making it a useful tool in cell biology. I >> would guess that many of us on this list know one or more of these >> people personally, and all of us have carried out research which >> they have made possible. It is a tremendous honour, but one which >> is well deserved, and I think that all of us in this community >> would like to join in offering our congratulations. >> >> >> Guy >> >> Ammasi Periasamy, Ph.D. >> >> Director, Keck Center for Cellular Imaging (KCCI) >> >> Professor of Biology and Biomedical Engineering >> >> Biology, Gilmer Hall (064), McCormick Rd >> >> University of Virginia >> >> Charlottesville, VA 22904 >> >> Voice: 434-243-7602 (Office); 982-4869 (lab) >> >> Fax:434-982-5210; Email:[hidden email] >> >> http//:www.kcci.virginia.edu >> >> ************************ >> >> Workshop on FRET Microscopy, March 3-7, 2009 >> >> http://www.kcci.virginia.edu/workshop/workshop2009/index.php >> >> ************************* >> >> ----------------------------------------------------- Resistere, Resistere, Resistere! Hold out, Hold out, Hold out! ----------------------------------------------------- Alberto Diaspro Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy fax +39-010314218 - tel +39 0103536426/309; email: [hidden email] - URL: www.lambs.it; THINK EBSA2009! www.ebsa2009.org ---------------------------------------------- |
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In reply to this post by Peter Carroll
Marty (now Dr. Chalfie I guess!) a few years back, told me he gave out
something like over 16,000 vials of GFP free before he exhausted his resources and finally arranged for someone else to ship it at some minimal fee. The new arrangement he jokingly remarked made him a hundredaire! Modest, generous, brilliant, and well deserving of this award! Mike Ignatius -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Peter Carroll Sent: Wednesday, October 08, 2008 7:51 AM To: [hidden email] Subject: Re: Nobel Prize for GFP I took a course in college with Chalfie, and never knew of his lab's involvement with GFP, something I now work with every day! Thanks for the heads-up, Guy. Periasamy, Ammasi (ap3t) wrote: > > I agree with Guy Cox. They deserve this award even though it took some > time to recognize their contribution. > > The investigation of biological system using imaging technology > tremendously improved because of their contribution. > > Congratulations!! > > > > Ammasi > > > > > > > > *From:* Confocal Microscopy List > [mailto:[hidden email]] *On Behalf Of *Guy Cox > *Sent:* Wednesday, October 08, 2008 8:22 AM > *To:* [hidden email] > *Subject:* Nobel Prize for GFP > > > > This year's Nobel Prize for Chemistry has been awarded to Osamu > Shimomura, Martin Chalfie and Roger Tsien for their work on > discovering GFP and making it a useful tool in cell biology. I would > guess that many of us on this list know one or more of these people > personally, and all of us have carried out research which they have > made possible. It is a tremendous honour, but one which is well > deserved, and I think that all of us in this community would like to > join in offering our congratulations. > > > > > Guy > > Ammasi Periasamy, Ph.D. > > Director, Keck Center for Cellular Imaging (KCCI) > > Professor of Biology and Biomedical Engineering > > Biology, Gilmer Hall (064), McCormick Rd > > University of Virginia > > Charlottesville, VA 22904 > > Voice: 434-243-7602 (Office); 982-4869 (lab) > > Fax:434-982-5210; Email:[hidden email] > > http//:www.kcci.virginia.edu > > ************************ > > Workshop on FRET Microscopy, March 3-7, 2009 > > http://www.kcci.virginia.edu/workshop/workshop2009/index.php > > ************************* > > > |
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It's nice to have some of "our own" win an honor of this
distinction... long overdue!
Congratulations! Barbara Foster, President Microscopy/Microscopy Education 7101 Royal Glen Trail, Suite A McKinney TX 75070 P: (972)924-5310 Skype: fostermme W: www.MicroscopyEducation.com uote. At 10:38 AM 10/8/2008, you wrote: Marty (now Dr. Chalfie I guess!) a few years back, told me he gave out |
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Patrick Van Oostveldt |
Re: Nobel Prize for GFP
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In reply to this post by lechristophe
Dear,
Indeed, great news for imaging people. Of cause we could ask if the impact of the GFP technology would have reached the same impact if imaging technology, certainly confocal microscopy, was not available to these people? Zernike obtained this price for phase contrast microscopy, would confocal microscopy have the same merrits? I really don't like to reduce the merits of these people but it shows clearly we need each other every day. With my sincere congratulations for all. Patrick Van Oostveldt Quoting Christophe Leterrier <[hidden email]>: > Great news ! I also have a thought for Douglas Prasher, because he cloned it > and first had the idea of making fusion proteins, but went out of business > just after publishing the sequence because of a lack of grants. > http://www.conncoll.edu/ccacad/zimmer/GFP-ww/prasher.html > > Christophe > > On Wed, Oct 8, 2008 at 16:05, Periasamy, Ammasi (ap3t) < > [hidden email]> wrote: > >> I agree with Guy Cox. They deserve this award even though it took some >> time to recognize their contribution. >> >> The investigation of biological system using imaging technology >> tremendously improved because of their contribution. >> >> Congratulations!! >> >> >> >> Ammasi >> >> >> >> >> >> >> >> *From:* Confocal Microscopy List [mailto:[hidden email]] >> *On Behalf Of *Guy Cox >> *Sent:* Wednesday, October 08, 2008 8:22 AM >> *To:* [hidden email] >> *Subject:* Nobel Prize for GFP >> >> >> >> This year's Nobel Prize for Chemistry has been awarded to Osamu Shimomura, >> Martin Chalfie and Roger Tsien for their work on discovering GFP and making >> it a useful tool in cell biology. I would guess that many of us on this >> list know one or more of these people personally, and all of us have carried >> out research which they have made possible. It is a tremendous honour, but >> one which is well deserved, and I think that all of us in this community >> would like to join in offering our congratulations. >> >> >> >> >> Guy >> >> Ammasi Periasamy, Ph.D. >> >> Director, Keck Center for Cellular Imaging (KCCI) >> >> Professor of Biology and Biomedical Engineering >> >> Biology, Gilmer Hall (064), McCormick Rd >> >> University of Virginia >> >> Charlottesville, VA 22904 >> >> Voice: 434-243-7602 (Office); 982-4869 (lab) >> >> Fax:434-982-5210; Email:[hidden email] <Email%[hidden email]> >> >> http//:www.kcci.virginia.edu >> >> ************************ >> >> Workshop on FRET Microscopy, March 3-7, 2009 >> >> http://www.kcci.virginia.edu/workshop/workshop2009/index.php >> >> ************************* >> >> >> > -- Dep. Moleculaire Biotechnologie Coupure links 653 B 9000 GENT tel 09 264 5969 fax 09 264 6219 |
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To those interested, NPR broadcast this morning, "Gene's discoverer Left Out of Nobel Prize" featured Douglas Prasher.
Jerry On Oct 9, 2008, at 10:27 AM, Patrick Van Oostveldt wrote:
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Jerry Calvin wrote:
> To those interested, NPR broadcast this morning, "Gene's discoverer Left > Out of Nobel Prize" featured Douglas Prasher. > http://www.npr.org/templates/story/story.php?storyId=95545761 Based on the interview, Dr. Prasher sounds like a remarkably generous soul. --Anybody got a job for the guy??? Martin -- Martin Wessendorf, Ph.D. office: (612) 626-0145 Assoc Prof, Dept Neuroscience lab: (612) 624-2991 University of Minnesota Preferred FAX: (612) 624-8118 6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009 Minneapolis, MN 55455 **MY E-MAIL ADDRESS HAS CHANGED. PLEASE USE [hidden email] ** |
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also sounds like someone who could easily work with others and would
enjoy the personal interactions. On Oct 9, 2008, at 11:30 AM, Martin Wessendorf wrote: > Jerry Calvin wrote: >> To those interested, NPR broadcast this morning, "Gene's discoverer >> Left Out of Nobel Prize" featured Douglas Prasher. >> http://www.npr.org/templates/story/story.php?storyId=95545761 > > Based on the interview, Dr. Prasher sounds like a remarkably > generous soul. --Anybody got a job for the guy??? > > Martin > -- > Martin Wessendorf, Ph.D. office: (612) 626-0145 > Assoc Prof, Dept Neuroscience lab: (612) 624-2991 > University of Minnesota Preferred FAX: (612) 624-8118 > 6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009 > Minneapolis, MN 55455 > **MY E-MAIL ADDRESS HAS CHANGED. PLEASE USE [hidden email] ** |
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In reply to this post by lechristophe
I agree. I know Dr. Prashar and hope that the NPR story makes him get
back to science. He still has plenty of brilliant discoveries for science. -Prabhakar Christophe Leterrier wrote: > Great news ! I also have a thought for Douglas Prasher, because he > cloned it and first had the idea of making fusion proteins, but went > out of business just after publishing the sequence because of a lack > of grants. > http://www.conncoll.edu/ccacad/zimmer/GFP-ww/prasher.html > > Christophe > > On Wed, Oct 8, 2008 at 16:05, Periasamy, Ammasi (ap3t) > <[hidden email] <mailto:[hidden email]>> wrote: > > I agree with Guy Cox. They deserve this award even though it took > some time to recognize their contribution. > > The investigation of biological system using imaging technology > tremendously improved because of their contribution. > > Congratulations!! > > > > Ammasi > > > > > > > > *From:* Confocal Microscopy List > [mailto:[hidden email] > <mailto:[hidden email]>] *On Behalf Of *Guy Cox > *Sent:* Wednesday, October 08, 2008 8:22 AM > > *To:* [hidden email] > <mailto:[hidden email]> > *Subject:* Nobel Prize for GFP > > > > This year's Nobel Prize for Chemistry has been awarded to Osamu > Shimomura, Martin Chalfie and Roger Tsien for their work on > discovering GFP and making it a useful tool in cell biology. I > would guess that many of us on this list know one or more of these > people personally, and all of us have carried out research which > they have made possible. It is a tremendous honour, but one which > is well deserved, and I think that all of us in this community > would like to join in offering our congratulations. > > > > > Guy > > Ammasi Periasamy, Ph.D. > > Director, Keck Center for Cellular Imaging (KCCI) > > Professor of Biology and Biomedical Engineering > > Biology, Gilmer Hall (064), McCormick Rd > > University of Virginia > > Charlottesville, VA 22904 > > Voice: 434-243-7602 (Office); 982-4869 (lab) > > Fax:434-982-5210; Email:[hidden email] > <mailto:Email%[hidden email]> > > http//:www.kcci.virginia.edu <http://www.kcci.virginia.edu> > > ************************ > > Workshop on FRET Microscopy, March 3-7, 2009 > > http://www.kcci.virginia.edu/workshop/workshop2009/index.php > > ************************* > > > > -- |
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B. Prabhakar Pandian |
Adherent Cell thickness/Height
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In reply to this post by Patrick Van Oostveldt
Hello:
Does anybody have information on the thickness of adherent cells. I am trying to figure out what is the typically height of a cell grown on a cover slip. Thanks, -Prabhakar |
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Csúcs Gábor |
Re: Adherent Cell thickness/Height
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Dear Prabhakar,
There is (unfortunately) no general answer to your question. The thickness can range from 1-2 microns up to 10-15 microns (perhaps even more...) depending on the used cell type. Furthermore, it is also cell cycle dependent: during mitosis (some) cells tend to round up (= are much thicker)... Greetings Gabor > Hello: > Does anybody have information on the thickness of adherent > cells. I am trying to figure out what is the typically height of a > cell grown on a cover slip. > > Thanks, > > -Prabhakar -- Gabor Csucs Light Microscopy Centre, ETH Zurich Schafmattstrasse 18, HPM F16 CH-8093, Zurich, Switzerland Web: www.lmc.ethz.ch Phone: +41 44 633 6221 Fax: +41 44 632 1298 e-mail: [hidden email] |
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Farid Jalali |
Re: Adherent Cell thickness/Height
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In reply to this post by B. Prabhakar Pandian
Hello Prabhakar
Agree with Gabor. Normal diploid human fibroblasts that have been parafromaldehye fixed and stained tend to be about 6-10um in thickness. Malignant cells will tend to be thicker due to aneuploidy/ generally greater DNA content. Mitotic cells are noticeably thicker as a result of rounding-up, but S and G2 cells will also tend to be somewhat thicker than G1 cells. Best Farid On Mon, Oct 27, 2008 at 1:06 PM, B. Prabhakar Pandian <[hidden email]> wrote: Hello: -- Farid Jalali MSc Program Leader- Cellular Imaging Core Applied Molecular Oncology and Radiation Medicine Program Princess Margaret Hospital (University Health Network) Toronto Medical Discovery Tower Toronto, Canada 416-581-7754 STTARR at TMDT 416-581-7791 STTARR Microscopy Suite |
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Nicholas Geisse |
Re: Adherent Cell thickness/Height
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In reply to this post by B. Prabhakar Pandian
Hi Prabhakar,
Of course, as others on the board have mentioned, the answer depends on various factors, so it's hard to come up with a solid answer. Using an atomic force microscope to measure several cell types, you can also see great variety on live cells (confirmed with and close to values obtained via confocal Z stacks on similarly prepared but fixed cells). For example, well-spread, non-confluent human and rat fibroblasts can take a 'fried egg' like appearance on a flat substrate, with the flatter parts being 1 um or less in Z, and the nucleus being 4-5 um in Z. I'd say it's been rare for me to see measurements above 8 um, but I primarily image well adhered fibroblasts and muscle cells that have lots of room to spread around. Sorry to sound so vague, but I think there will be a lot of variety out there... Nick _____________________ Nicholas Geisse, PhD Asylum Research 805-696-6466 x 309 805-696-6444 (fax) On 10/27/08 10:06 AM, "B. Prabhakar Pandian" <[hidden email]> wrote: > Hello: > Does anybody have information on the thickness of adherent > cells. I am trying to figure out what is the typically height of a cell > grown on a cover slip. > > Thanks, > > -Prabhakar ------ End of Forwarded Message |
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B. Prabhakar Pandian |
Low Flow Peristaltic Pump
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In reply to this post by Martin Wessendorf-2
Hello:
Does anybody know of a peristaltic pump that can go less than 1ul/min. I am looking for one that can work ideally around 0.1-0.5 ul/min. Even lower would be great. Thanks, -Prabhakar |
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Grimm, Deborah A |
Re: Low Flow Peristaltic Pump
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I think you would have better luck looking for a syringe pump.
Deborah Grimm Coordinated Instrumentation Facility Tulane University -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of B. Prabhakar Pandian Sent: Thursday, November 06, 2008 12:13 PM To: [hidden email] Subject: Low Flow Peristaltic Pump Hello: Does anybody know of a peristaltic pump that can go less than 1ul/min. I am looking for one that can work ideally around 0.1-0.5 ul/min. Even lower would be great. Thanks, -Prabhakar |
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