sealing glue on dipping objective chipped off

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mcammer mcammer
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sealing glue on dipping objective chipped off

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Leica added a sealing ring of glue to their 40X N.A. 0.80 water dipping objective and some of it has chipped off.

Picture at http://www.flickr.com/photos/mcammer/9240581316/

We were thinking of sealing it with layers of Krazy glue (after covering the lens element to protect from the vapor), high grade silicon caulk, or 5 minute epoxy.

Anyone have experience with this, or should we have Leica fix it (probably more expensive)?

Thank you!!

________________________________________________________
Michael Cammer, Assistant Research Scientist
Microscopy Core, NYU Langone Medical Center & Skirball Institute of Biomolecular Medicine
Cell: (914) 309-3270   Microscopy Lab: (212) 263-7099   Dustin Lab: (212) 263-3208
Gabriel Lapointe-4 Gabriel Lapointe-4
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Re: sealing glue on dipping objective chipped off

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Hi Micheal,
I don't have that objective, is the seal flexible or hard. If it has some
flexibility or it feels rubbery I would go with a high quality bathroom or
even better fish tank silicone. If it's hard I would go with epoxy. In any
case, since i't far away from the lens I would try to do the repair myself
to save so money.

One note though, I don't know if there is a gap between the two part that
need sealing. You might need to be careful to make sure any sealant you use
doesn't get inside. Personally, I would flip the objective nose down as
soon as the surface of the sealant stops being liquid. Epoxy solidify
through a chemical reaction so in theory it's going to harden evenly.
However, silicone harden outside-in, so the centre can stay liquid for
quite some time after it looks solid. In this situation I would be afraid
that some of it find it's way inside the lens tube.


Good luck,

*Gabriel Lapointe, M.Sc.*
Lab Manager / Microscopy Specialist
Concordia University, Biology Department
7141 Sherbrooke St. West SP 534
Montréal QC H4B 1R6 Canada
Lab : (514) 848-2424 x5988
Office : (514) 848-2424 x3008
Fax : (514) 848-2881
Cell : (514) 278-0247
[hidden email]
cmac.concordia.ca
http://gabriellapointe.ca


2013/7/8 Cammer, Michael <[hidden email]>

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Leica added a sealing ring of glue to their 40X N.A. 0.80 water dipping
> objective and some of it has chipped off.
>
> Picture at http://www.flickr.com/photos/mcammer/9240581316/
>
> We were thinking of sealing it with layers of Krazy glue (after covering
> the lens element to protect from the vapor), high grade silicon caulk, or 5
> minute epoxy.
>
> Anyone have experience with this, or should we have Leica fix it (probably
> more expensive)?
>
> Thank you!!
>
> ________________________________________________________
> Michael Cammer, Assistant Research Scientist
> Microscopy Core, NYU Langone Medical Center & Skirball Institute of
> Biomolecular Medicine
> Cell: (914) 309-3270   Microscopy Lab: (212) 263-7099   Dustin Lab: (212)
> 263-3208
>
Craig Brideau Craig Brideau
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Re: sealing glue on dipping objective chipped off

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To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dow Corning 3140 MIL-A- 46146 RTV Coating works well. It is a relatively
biocompatible sealing silicone. We use it for chambers and I have used it
to repair a chipped lens.

Craig
On 2013-07-08 9:53 AM, "Gabriel Lapointe" <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi Micheal,
> I don't have that objective, is the seal flexible or hard. If it has some
> flexibility or it feels rubbery I would go with a high quality bathroom or
> even better fish tank silicone. If it's hard I would go with epoxy. In any
> case, since i't far away from the lens I would try to do the repair myself
> to save so money.
>
> One note though, I don't know if there is a gap between the two part that
> need sealing. You might need to be careful to make sure any sealant you use
> doesn't get inside. Personally, I would flip the objective nose down as
> soon as the surface of the sealant stops being liquid. Epoxy solidify
> through a chemical reaction so in theory it's going to harden evenly.
> However, silicone harden outside-in, so the centre can stay liquid for
> quite some time after it looks solid. In this situation I would be afraid
> that some of it find it's way inside the lens tube.
>
>
> Good luck,
>
> *Gabriel Lapointe, M.Sc.*
> Lab Manager / Microscopy Specialist
> Concordia University, Biology Department
> 7141 Sherbrooke St. West SP 534
> Montréal QC H4B 1R6 Canada
> Lab : (514) 848-2424 x5988
> Office : (514) 848-2424 x3008
> Fax : (514) 848-2881
> Cell : (514) 278-0247
> [hidden email]
> cmac.concordia.ca
> http://gabriellapointe.ca
>
>
> 2013/7/8 Cammer, Michael <[hidden email]>
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Leica added a sealing ring of glue to their 40X N.A. 0.80 water dipping
> > objective and some of it has chipped off.
> >
> > Picture at http://www.flickr.com/photos/mcammer/9240581316/
> >
> > We were thinking of sealing it with layers of Krazy glue (after covering
> > the lens element to protect from the vapor), high grade silicon caulk,
> or 5
> > minute epoxy.
> >
> > Anyone have experience with this, or should we have Leica fix it
> (probably
> > more expensive)?
> >
> > Thank you!!
> >
> > ________________________________________________________
> > Michael Cammer, Assistant Research Scientist
> > Microscopy Core, NYU Langone Medical Center & Skirball Institute of
> > Biomolecular Medicine
> > Cell: (914) 309-3270   Microscopy Lab: (212) 263-7099   Dustin Lab: (212)
> > 263-3208
> >
>
Chris Tully Chris Tully
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Re: sealing glue on dipping objective chipped off

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Even though I might try the repair myself, I would at least call the local
repair guy and ask what is used to make the original seal and if there are
any recommendations for replacing the sealant.  The main reason for asking
is to insure that the new sealant is compatible with the old, and has the
required properties for the objective to continue to function properly.

Also, you might want to consider removing all of the old sealant since it
has started to chip off. Don't forget that ALL surfaces that the new
sealant is expected to cling to must be clean of free of debris, oil,
water, etc. Regardless of the surfaces being sealed together (window jambs,
car engines, scientific apparatus) every instruction set I have ever read
strongly recommends removing as much of the old seal as possible to insure
that the new sealant has a solid, clean surface to bond with.

Finally, keep in mind that doing his sort of repair yourself will void any
warranty that may still cover the objective.

Chris Tully
Microscopy and Image Analysis Expert
[hidden email]
[hidden email]
www.ImageIncyte.com
240-475-9753 (c)

[image: View my profile on LinkedIn]<http://www.linkedin.com/in/christully/>


On Mon, Jul 8, 2013 at 3:15 PM, Craig Brideau <[hidden email]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dow Corning 3140 MIL-A- 46146 RTV Coating works well. It is a relatively
> biocompatible sealing silicone. We use it for chambers and I have used it
> to repair a chipped lens.
>
> Craig
> On 2013-07-08 9:53 AM, "Gabriel Lapointe" <[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Hi Micheal,
> > I don't have that objective, is the seal flexible or hard. If it has some
> > flexibility or it feels rubbery I would go with a high quality bathroom
> or
> > even better fish tank silicone. If it's hard I would go with epoxy. In
> any
> > case, since i't far away from the lens I would try to do the repair
> myself
> > to save so money.
> >
> > One note though, I don't know if there is a gap between the two part that
> > need sealing. You might need to be careful to make sure any sealant you
> use
> > doesn't get inside. Personally, I would flip the objective nose down as
> > soon as the surface of the sealant stops being liquid. Epoxy solidify
> > through a chemical reaction so in theory it's going to harden evenly.
> > However, silicone harden outside-in, so the centre can stay liquid for
> > quite some time after it looks solid. In this situation I would be afraid
> > that some of it find it's way inside the lens tube.
> >
> >
> > Good luck,
> >
> > *Gabriel Lapointe, M.Sc.*
> > Lab Manager / Microscopy Specialist
> > Concordia University, Biology Department
> > 7141 Sherbrooke St. West SP 534
> > Montréal QC H4B 1R6 Canada
> > Lab : (514) 848-2424 x5988
> > Office : (514) 848-2424 x3008
> > Fax : (514) 848-2881
> > Cell : (514) 278-0247
> > [hidden email]
> > cmac.concordia.ca
> > http://gabriellapointe.ca
> >
> >
> > 2013/7/8 Cammer, Michael <[hidden email]>
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > *****
> > >
> > > Leica added a sealing ring of glue to their 40X N.A. 0.80 water dipping
> > > objective and some of it has chipped off.
> > >
> > > Picture at http://www.flickr.com/photos/mcammer/9240581316/
> > >
> > > We were thinking of sealing it with layers of Krazy glue (after
> covering
> > > the lens element to protect from the vapor), high grade silicon caulk,
> > or 5
> > > minute epoxy.
> > >
> > > Anyone have experience with this, or should we have Leica fix it
> > (probably
> > > more expensive)?
> > >
> > > Thank you!!
> > >
> > > ________________________________________________________
> > > Michael Cammer, Assistant Research Scientist
> > > Microscopy Core, NYU Langone Medical Center & Skirball Institute of
> > > Biomolecular Medicine
> > > Cell: (914) 309-3270   Microscopy Lab: (212) 263-7099   Dustin Lab:
> (212)
> > > 263-3208
> > >
> >
>