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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Leica added a sealing ring of glue to their 40X N.A. 0.80 water dipping objective and some of it has chipped off. Picture at http://www.flickr.com/photos/mcammer/9240581316/ We were thinking of sealing it with layers of Krazy glue (after covering the lens element to protect from the vapor), high grade silicon caulk, or 5 minute epoxy. Anyone have experience with this, or should we have Leica fix it (probably more expensive)? Thank you!! ________________________________________________________ Michael Cammer, Assistant Research Scientist Microscopy Core, NYU Langone Medical Center & Skirball Institute of Biomolecular Medicine Cell: (914) 309-3270 Microscopy Lab: (212) 263-7099 Dustin Lab: (212) 263-3208 |
Gabriel Lapointe-4 |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi Micheal, I don't have that objective, is the seal flexible or hard. If it has some flexibility or it feels rubbery I would go with a high quality bathroom or even better fish tank silicone. If it's hard I would go with epoxy. In any case, since i't far away from the lens I would try to do the repair myself to save so money. One note though, I don't know if there is a gap between the two part that need sealing. You might need to be careful to make sure any sealant you use doesn't get inside. Personally, I would flip the objective nose down as soon as the surface of the sealant stops being liquid. Epoxy solidify through a chemical reaction so in theory it's going to harden evenly. However, silicone harden outside-in, so the centre can stay liquid for quite some time after it looks solid. In this situation I would be afraid that some of it find it's way inside the lens tube. Good luck, *Gabriel Lapointe, M.Sc.* Lab Manager / Microscopy Specialist Concordia University, Biology Department 7141 Sherbrooke St. West SP 534 Montréal QC H4B 1R6 Canada Lab : (514) 848-2424 x5988 Office : (514) 848-2424 x3008 Fax : (514) 848-2881 Cell : (514) 278-0247 [hidden email] cmac.concordia.ca http://gabriellapointe.ca 2013/7/8 Cammer, Michael <[hidden email]> > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Leica added a sealing ring of glue to their 40X N.A. 0.80 water dipping > objective and some of it has chipped off. > > Picture at http://www.flickr.com/photos/mcammer/9240581316/ > > We were thinking of sealing it with layers of Krazy glue (after covering > the lens element to protect from the vapor), high grade silicon caulk, or 5 > minute epoxy. > > Anyone have experience with this, or should we have Leica fix it (probably > more expensive)? > > Thank you!! > > ________________________________________________________ > Michael Cammer, Assistant Research Scientist > Microscopy Core, NYU Langone Medical Center & Skirball Institute of > Biomolecular Medicine > Cell: (914) 309-3270 Microscopy Lab: (212) 263-7099 Dustin Lab: (212) > 263-3208 > |
Craig Brideau |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Dow Corning 3140 MIL-A- 46146 RTV Coating works well. It is a relatively biocompatible sealing silicone. We use it for chambers and I have used it to repair a chipped lens. Craig On 2013-07-08 9:53 AM, "Gabriel Lapointe" <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi Micheal, > I don't have that objective, is the seal flexible or hard. If it has some > flexibility or it feels rubbery I would go with a high quality bathroom or > even better fish tank silicone. If it's hard I would go with epoxy. In any > case, since i't far away from the lens I would try to do the repair myself > to save so money. > > One note though, I don't know if there is a gap between the two part that > need sealing. You might need to be careful to make sure any sealant you use > doesn't get inside. Personally, I would flip the objective nose down as > soon as the surface of the sealant stops being liquid. Epoxy solidify > through a chemical reaction so in theory it's going to harden evenly. > However, silicone harden outside-in, so the centre can stay liquid for > quite some time after it looks solid. In this situation I would be afraid > that some of it find it's way inside the lens tube. > > > Good luck, > > *Gabriel Lapointe, M.Sc.* > Lab Manager / Microscopy Specialist > Concordia University, Biology Department > 7141 Sherbrooke St. West SP 534 > Montréal QC H4B 1R6 Canada > Lab : (514) 848-2424 x5988 > Office : (514) 848-2424 x3008 > Fax : (514) 848-2881 > Cell : (514) 278-0247 > [hidden email] > cmac.concordia.ca > http://gabriellapointe.ca > > > 2013/7/8 Cammer, Michael <[hidden email]> > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > Leica added a sealing ring of glue to their 40X N.A. 0.80 water dipping > > objective and some of it has chipped off. > > > > Picture at http://www.flickr.com/photos/mcammer/9240581316/ > > > > We were thinking of sealing it with layers of Krazy glue (after covering > > the lens element to protect from the vapor), high grade silicon caulk, > or 5 > > minute epoxy. > > > > Anyone have experience with this, or should we have Leica fix it > (probably > > more expensive)? > > > > Thank you!! > > > > ________________________________________________________ > > Michael Cammer, Assistant Research Scientist > > Microscopy Core, NYU Langone Medical Center & Skirball Institute of > > Biomolecular Medicine > > Cell: (914) 309-3270 Microscopy Lab: (212) 263-7099 Dustin Lab: (212) > > 263-3208 > > > |
Chris Tully |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Even though I might try the repair myself, I would at least call the local repair guy and ask what is used to make the original seal and if there are any recommendations for replacing the sealant. The main reason for asking is to insure that the new sealant is compatible with the old, and has the required properties for the objective to continue to function properly. Also, you might want to consider removing all of the old sealant since it has started to chip off. Don't forget that ALL surfaces that the new sealant is expected to cling to must be clean of free of debris, oil, water, etc. Regardless of the surfaces being sealed together (window jambs, car engines, scientific apparatus) every instruction set I have ever read strongly recommends removing as much of the old seal as possible to insure that the new sealant has a solid, clean surface to bond with. Finally, keep in mind that doing his sort of repair yourself will void any warranty that may still cover the objective. Chris Tully Microscopy and Image Analysis Expert [hidden email] [hidden email] www.ImageIncyte.com 240-475-9753 (c) [image: View my profile on LinkedIn]<http://www.linkedin.com/in/christully/> On Mon, Jul 8, 2013 at 3:15 PM, Craig Brideau <[hidden email]>wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Dow Corning 3140 MIL-A- 46146 RTV Coating works well. It is a relatively > biocompatible sealing silicone. We use it for chambers and I have used it > to repair a chipped lens. > > Craig > On 2013-07-08 9:53 AM, "Gabriel Lapointe" <[hidden email]> > wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > ***** > > > > Hi Micheal, > > I don't have that objective, is the seal flexible or hard. If it has some > > flexibility or it feels rubbery I would go with a high quality bathroom > or > > even better fish tank silicone. If it's hard I would go with epoxy. In > any > > case, since i't far away from the lens I would try to do the repair > myself > > to save so money. > > > > One note though, I don't know if there is a gap between the two part that > > need sealing. You might need to be careful to make sure any sealant you > use > > doesn't get inside. Personally, I would flip the objective nose down as > > soon as the surface of the sealant stops being liquid. Epoxy solidify > > through a chemical reaction so in theory it's going to harden evenly. > > However, silicone harden outside-in, so the centre can stay liquid for > > quite some time after it looks solid. In this situation I would be afraid > > that some of it find it's way inside the lens tube. > > > > > > Good luck, > > > > *Gabriel Lapointe, M.Sc.* > > Lab Manager / Microscopy Specialist > > Concordia University, Biology Department > > 7141 Sherbrooke St. West SP 534 > > Montréal QC H4B 1R6 Canada > > Lab : (514) 848-2424 x5988 > > Office : (514) 848-2424 x3008 > > Fax : (514) 848-2881 > > Cell : (514) 278-0247 > > [hidden email] > > cmac.concordia.ca > > http://gabriellapointe.ca > > > > > > 2013/7/8 Cammer, Michael <[hidden email]> > > > > > ***** > > > To join, leave or search the confocal microscopy listserv, go to: > > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > > ***** > > > > > > Leica added a sealing ring of glue to their 40X N.A. 0.80 water dipping > > > objective and some of it has chipped off. > > > > > > Picture at http://www.flickr.com/photos/mcammer/9240581316/ > > > > > > We were thinking of sealing it with layers of Krazy glue (after > covering > > > the lens element to protect from the vapor), high grade silicon caulk, > > or 5 > > > minute epoxy. > > > > > > Anyone have experience with this, or should we have Leica fix it > > (probably > > > more expensive)? > > > > > > Thank you!! > > > > > > ________________________________________________________ > > > Michael Cammer, Assistant Research Scientist > > > Microscopy Core, NYU Langone Medical Center & Skirball Institute of > > > Biomolecular Medicine > > > Cell: (914) 309-3270 Microscopy Lab: (212) 263-7099 Dustin Lab: > (212) > > > 263-3208 > > > > > > |
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