searching for 405 laser photoactivatable dye

[Rietdorf, Jens]

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Dear list,

 

I am looking for a fluorescent dye that I could couple to a protein toxin. After incubation with some cells, I want to photo-activate the dye by irradiation with a laser on the confocal. Unfortunately, we dont have a real UV but only 405nm lasers (or 444,476,488,514,543,561,594,633,647). Any suggestions for a suitable dye? Of course we could produce a FP-fusion of the toxin, but is there a simple dye that can do the job?

Thanks & regards, jens

 

---

Dr. Jens Rietdorf[hidden email]
Head Microscopy
Novartis Research Foundation
Friedrich-Miescher-Institute, wro1066.2.32
Maulbeerstr.66, CH-4058 Basel, Switzerland
phone [phone] mobil [phone]
Email:rietdorf(at)fmi.ch

 

[Klara Balint]

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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Jens,
I would suggest you to visit www.Invitrogen.com

They have every kind of dyes and the inforation you need.

Klara
Quoting "Rietdorf, Jens" <[hidden email]>:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear list,
>  
> I am looking for a fluorescent dye that I could couple to a protein
> toxin. After incubation with some cells, I want to photo-activate the
> dye by irradiation with a laser on the confocal. Unfortunately, we dont
> have a real UV but only 405nm lasers (or
> 444,476,488,514,543,561,594,633,647). Any suggestions for a suitable
> dye? Of course we could produce a FP-fusion of the toxin, but is there a
> simple dye that can do the job?
>
> Thanks & regards, jens
>
>  
>
> ---
>
> Dr. Jens Rietdorf <mailto:[hidden email]>
> Head Microscopy
> Novartis Research Foundation
> Friedrich-Miescher-Institute <http://www.fmi.ch/> , wro1066.2.32
> Maulbeerstr.66, CH-4058 Basel, Switzerland
> phone [phone] mobil [phone]
> Email:rietdorf(at)fmi.ch
>
>  
>

--
Klara Balint, MD
Postdoctoral researcher
dr.George Coukos`s Laboratory
Center for Research on Reproduction and Women`s Health
University of Pennsylvania
421 Curie Blvd, BRB II/III. Building, Room 1331.
Philadelphia, PA 19104
Phone: [phone]