searching for Hamamatsu Orca ER C4742-80-12AG

[Owens, Peter]

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*****

Dear all,

We are searching for a replacement Hamamatsu Orca ER C4742-80-12AG for one of our systems. Our old one stopped working recently and due to its age is not worth repairing. We would like to replace it in the short term rather than incurring the cost of buying a new one along with upgrading the imaging software.

Does anyone out there have one that they would be willing to sell or know of where we could find one?

Thanks,

Peter Owens


_______________________________
Centre for Microscopy and Imaging NUIG
National University of Ireland Galway
www.imaging.nuigalway.ie
www.nbipireland.ie
Tel:[phone]







-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Neil Kad
Sent: 21 October 2014 19:23
To: [hidden email]
Subject: Re: PSF fitting in widefield microscopy

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Try this Gaussian fitter, if I understand correctly what you are trying to achieve this will work straight from ImageJ.

http://kadlab.demojoomla.com/index.php/links-and-downloads/9-downloads

Choose Gaussian Fitter





 



Cheers,

 

Neil

 

=================================

Neil M. Kad PhD.,

School of Biosciences,

University of Kent,

Canterbury,

Kent CT2 7NH,

United Kingdom.

 

Tel: [phone]

=================================

> Date: Tue, 21 Oct 2014 11:53:52 -0500
> From: [hidden email]
> Subject: PSF fitting in widefield microscopy
> To: [hidden email]
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear all,
>
> Could you please recommend a free software (and a manual for it) to
> perform the following task?
>
> I have an image of microtubules. For each microtubule, I drew a ROI
> around corresponding microtubule, 7 pixels wide, and decomposed it
> into series of 1x7 pixel "images" of microtubule cross-section. I
> would like to fit PSF for given optical condition to each of
> cross-section and obtain the values: goodness-of- fit, estimated maximum value, and estimated maximum position.
>
> Yes, it is like STORM, but I don't know if software presented in
> Zhuang Research Lab homepage suits the task.
>
> Best,
> Sergey

     

[Csúcs Gábor-3]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Peter,

Do you look only for the camera or the controller unit or for both?

Greetings     Gabor


On 10/29/14 3:32 PM, "Owens, Peter" <[hidden email]> wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>Post images on http://www.imgur.com and include the link in your posting.
>*****
>
>Dear all,
>
>We are searching for a replacement Hamamatsu Orca ER C4742-80-12AG for
>one of our systems. Our old one stopped working recently and due to its
>age is not worth repairing. We would like to replace it in the short term
>rather than incurring the cost of buying a new one along with upgrading
>the imaging software.
>
>Does anyone out there have one that they would be willing to sell or know
>of where we could find one?
>
>Thanks,
>
>Peter Owens
>
>
>_______________________________
>Centre for Microscopy and Imaging NUIG
>National University of Ireland Galway
>www.imaging.nuigalway.ie
>www.nbipireland.ie
>Tel:[phone]
>
>
>
>
>
>
>
>-----Original Message-----
>From: Confocal Microscopy List [mailto:[hidden email]]
>On Behalf Of Neil Kad
>Sent: 21 October 2014 19:23
>To: [hidden email]
>Subject: Re: PSF fitting in widefield microscopy
>
>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>Post images on http://www.imgur.com and include the link in your posting.
>*****
>
>Try this Gaussian fitter, if I understand correctly what you are trying
>to achieve this will work straight from ImageJ.
>
>http://kadlab.demojoomla.com/index.php/links-and-downloads/9-downloads
>
>Choose Gaussian Fitter
>
>
>
>
>
>
>
>
>
>Cheers,
>
>
>
>Neil
>
>
>
>=================================
>
>Neil M. Kad PhD.,
>
>School of Biosciences,
>
>University of Kent,
>
>Canterbury,
>
>Kent CT2 7NH,
>
>United Kingdom.
>
>
>
>Tel: [phone]
>
>=================================
>
>
>
>> Date: Tue, 21 Oct 2014 11:53:52 -0500
>> From: [hidden email]
>> Subject: PSF fitting in widefield microscopy
>> To: [hidden email]
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your
>>posting.
>> *****
>>
>> Dear all,
>>
>> Could you please recommend a free software (and a manual for it) to
>> perform the following task?
>>
>> I have an image of microtubules. For each microtubule, I drew a ROI
>> around corresponding microtubule, 7 pixels wide, and decomposed it
>> into series of 1x7 pixel "images" of microtubule cross-section. I
>> would like to fit PSF for given optical condition to each of
>> cross-section and obtain the values: goodness-of- fit, estimated
>>maximum value, and estimated maximum position.
>>
>> Yes, it is like STORM, but I don't know if software presented in
>> Zhuang Research Lab homepage suits the task.
>>
>> Best,
>> Sergey
>    

[Owens, Peter]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Gabor,

I just need the camera, not the controller.

Thanks

Peter

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Csúcs Gábor
Sent: 29 October 2014 14:39
To: [hidden email]
Subject: Re: searching for Hamamatsu Orca ER C4742-80-12AG

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Peter,

Do you look only for the camera or the controller unit or for both?

Greetings     Gabor


On 10/29/14 3:32 PM, "Owens, Peter" <[hidden email]> wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>Post images on http://www.imgur.com and include the link in your posting.
>*****
>
>Dear all,
>
>We are searching for a replacement Hamamatsu Orca ER C4742-80-12AG for
>one of our systems. Our old one stopped working recently and due to its
>age is not worth repairing. We would like to replace it in the short
>term rather than incurring the cost of buying a new one along with
>upgrading the imaging software.
>
>Does anyone out there have one that they would be willing to sell or
>know of where we could find one?
>
>Thanks,
>
>Peter Owens
>
>
>_______________________________
>Centre for Microscopy and Imaging NUIG
>National University of Ireland Galway
>www.imaging.nuigalway.ie
>www.nbipireland.ie
>Tel:[phone]
>
>
>
>
>
>
>
>-----Original Message-----
>From: Confocal Microscopy List
>[mailto:[hidden email]]
>On Behalf Of Neil Kad
>Sent: 21 October 2014 19:23
>To: [hidden email]
>Subject: Re: PSF fitting in widefield microscopy
>
>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>Post images on http://www.imgur.com and include the link in your posting.
>*****
>
>Try this Gaussian fitter, if I understand correctly what you are trying
>to achieve this will work straight from ImageJ.
>
>http://kadlab.demojoomla.com/index.php/links-and-downloads/9-downloads
>
>Choose Gaussian Fitter
>
>
>
>
>
>
>
>
>
>Cheers,
>
>
>
>Neil
>
>
>
>=================================
>
>Neil M. Kad PhD.,
>
>School of Biosciences,
>
>University of Kent,
>
>Canterbury,
>
>Kent CT2 7NH,
>
>United Kingdom.
>
>
>
>Tel: [phone]
>
>=================================
>
>
>
>> Date: Tue, 21 Oct 2014 11:53:52 -0500
>> From: [hidden email]
>> Subject: PSF fitting in widefield microscopy
>> To: [hidden email]
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your
>>posting.
>> *****
>>
>> Dear all,
>>
>> Could you please recommend a free software (and a manual for it) to
>> perform the following task?
>>
>> I have an image of microtubules. For each microtubule, I drew a ROI  
>>around corresponding microtubule, 7 pixels wide, and decomposed it  
>>into series of 1x7 pixel "images" of microtubule cross-section. I  
>>would like to fit PSF for given optical condition to each of  
>>cross-section and obtain the values: goodness-of- fit, estimated
>>maximum value, and estimated maximum position.
>>
>> Yes, it is like STORM, but I don't know if software presented in
>> Zhuang Research Lab homepage suits the task.
>>
>> Best,
>> Sergey
>    

[Melikian, Gregory-2]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Gabor,
We got Orca ER C4742-80 that was not used much (not sure about 12AG).
Let me know if you are interested.
Thanks,
Greg

Gregory Melikian, Ph.D.
Department of Pediatrics, Infectious Diseases
Emory University
2015 Uppergate Dr., Rm. 546
Atlanta, GA 30322
Tel:  [phone]
[hidden email]
Web: http://www.pediatrics.emory.edu/divisions/infectiousdisease/research/melikian/index.html



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Owens, Peter
Sent: Wednesday, October 29, 2014 11:28 AM
To: [hidden email]
Subject: Re: searching for Hamamatsu Orca ER C4742-80-12AG

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Gabor,

I just need the camera, not the controller.

Thanks

Peter

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Csúcs Gábor
Sent: 29 October 2014 14:39
To: [hidden email]
Subject: Re: searching for Hamamatsu Orca ER C4742-80-12AG

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Peter,

Do you look only for the camera or the controller unit or for both?

Greetings     Gabor


On 10/29/14 3:32 PM, "Owens, Peter" <[hidden email]> wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>Post images on http://www.imgur.com and include the link in your posting.
>*****
>
>Dear all,
>
>We are searching for a replacement Hamamatsu Orca ER C4742-80-12AG for
>one of our systems. Our old one stopped working recently and due to its
>age is not worth repairing. We would like to replace it in the short
>term rather than incurring the cost of buying a new one along with
>upgrading the imaging software.
>
>Does anyone out there have one that they would be willing to sell or
>know of where we could find one?
>
>Thanks,
>
>Peter Owens
>
>
>_______________________________
>Centre for Microscopy and Imaging NUIG
>National University of Ireland Galway
>www.imaging.nuigalway.ie
>www.nbipireland.ie
>Tel:[phone]
>
>
>
>
>
>
>
>-----Original Message-----
>From: Confocal Microscopy List
>[mailto:[hidden email]]
>On Behalf Of Neil Kad
>Sent: 21 October 2014 19:23
>To: [hidden email]
>Subject: Re: PSF fitting in widefield microscopy
>
>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>Post images on http://www.imgur.com and include the link in your posting.
>*****
>
>Try this Gaussian fitter, if I understand correctly what you are trying
>to achieve this will work straight from ImageJ.
>
>http://kadlab.demojoomla.com/index.php/links-and-downloads/9-downloads
>
>Choose Gaussian Fitter
>
>
>
>
>
>
>
>
>
>Cheers,
>
>
>
>Neil
>
>
>
>=================================
>
>Neil M. Kad PhD.,
>
>School of Biosciences,
>
>University of Kent,
>
>Canterbury,
>
>Kent CT2 7NH,
>
>United Kingdom.
>
>
>
>Tel: [phone]
>
>=================================
>
>
>
>> Date: Tue, 21 Oct 2014 11:53:52 -0500
>> From: [hidden email]
>> Subject: PSF fitting in widefield microscopy
>> To: [hidden email]
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your
>>posting.
>> *****
>>
>> Dear all,
>>
>> Could you please recommend a free software (and a manual for it) to
>> perform the following task?
>>
>> I have an image of microtubules. For each microtubule, I drew a ROI
>>around corresponding microtubule, 7 pixels wide, and decomposed it
>>into series of 1x7 pixel "images" of microtubule cross-section. I
>>would like to fit PSF for given optical condition to each of
>>cross-section and obtain the values: goodness-of- fit, estimated
>>maximum value, and estimated maximum position.
>>
>> Yes, it is like STORM, but I don't know if software presented in
>> Zhuang Research Lab homepage suits the task.
>>
>> Best,
>> Sergey
>

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