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I am currently measuring simultaltaneous bleedthrough signal as follows on a zeiss 880 equipped with a green sensitive pmt= chl1,
central single GaAsp = chl2 and red sensitive pmt=chl3.
The set up for dual imaging is as follows:
green emission signal (500-530 nm) is recorded on to the single GaAsP operated in photon counting mode (chl 2)
red emission signal is recorded on a red sensitive pmt operated in photon counting mode (chl 3)
The quantum efficiency of the 2 detectors is significantly different, is there any way to correct for the QE difference when measuring spillover. Any info would be helpful
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