splitting signals with Y probe

[Zhang, Yuwei]

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Dear colleagues,



I am working on disomy frequency with probes from Abbott. The signal is
very clear and figures are gorgeous. But I do see some splitting signals in
one cell quite frequently. Sometimes, there are filaments or some
connection between signals. Sometimes, the signals are equal to or less
than 1 probe width apart. My software, a customerized software developed in
Matlab, considers this as two spots so the rate of YY is higher.



I try to decrease the denature time.  But when the denature time is
decreased, I still see splitting signals. In addition, the hybridization
efficiency and other probe' signal strength  are affected.



If anyone has experience with  probes from Abbott, would you please give me
some advice.



Would you please let me know what I can do? Or do you have any better
software to recommend?



Thanks.





Yuwei Zhang PhD



Department of Environmental and Ocupational Health

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Room 112

Washington, DC 20052

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