stage top incubator

[leoncio vergara]

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Hi

I was wondering if anybody could tell me about their experience with the Live-
Cell stage top incubator from Pathology Devices Inc
(http://www.pathologydevices.com/LiveCell.htm) or the Oko-lab Electric
microscope stage incubator (http://www.oko-lab.com/39.page).

Both seem to be very similar. We are looking for a solution for a BSL2 facility
for live cell experiments on a Olympus DSU IX81 microscope with ZDCII. We
want to do experiments with specifically with Labtek-type chambered
coverglasses and 35 mm petri dishes (glass bottom).

I am looking for quality and reliability for temperature and environmental
control (CO2 and Humidity) as well as easy of use. For a multiuser facility like
ours, simplicity and robust design are good. For BSL2, the use of disposable
chambers is also desirable, even is we have to sacrifice some in terms of
accuracy, etc.

Thanks in advance for any information

Leoncio Vergara
UTMB, Galveston Texas

[abhijit debroy]

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Hi,

We have used the LiveCell chamber a lot with our confocals and with MatTek
35 mm diameter Glass bottom dishes. The chambers work very well. though it
takes quite a bit of time for the humidity to reach 75%. Also we usually
used vacuum grease to seal the glass bottom dish with the chamber. I am not
certain if there would be any issues if there were no seals.

I hope this was useful.

Thanks
Abhijit Deb Roy
Biomedical Engineering Program
University of Connecticut, Storrs Campus
PhD Candidate

On Tue, Apr 5, 2011 at 7:35 PM, Leoncio Vergara <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi
>
> I was wondering if anybody could tell me about their experience with the
> Live-
> Cell stage top incubator from Pathology Devices Inc
> (http://www.pathologydevices.com/LiveCell.htm) or the Oko-lab Electric
> microscope stage incubator (http://www.oko-lab.com/39.page).
>
> Both seem to be very similar. We are looking for a solution for a BSL2
> facility
> for live cell experiments on a Olympus DSU IX81 microscope with ZDCII. We
> want to do experiments with specifically with Labtek-type chambered
> coverglasses and 35 mm petri dishes (glass bottom).
>
> I am looking for quality and reliability for temperature and environmental
> control (CO2 and Humidity) as well as easy of use. For a multiuser facility
> like
> ours, simplicity and robust design are good. For BSL2, the use of
> disposable
> chambers is also desirable, even is we have to sacrifice some in terms of
> accuracy, etc.
>
> Thanks in advance for any information
>
> Leoncio Vergara
> UTMB, Galveston Texas
>

--
Abhijit Deb Roy
Biomedical Engineering Program
University of Connecticut, Storrs Campus
PhD Candidate

[Ryan Geil]

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I would encourage you to consider the Live Cell Instrument TC system available through Quorum.
It comes with specific adapters for both 35mm dishes and LabTek II chambers that are designed for optimal heat transfer.  The LCI systems heat from both the top and the bottom of the incubator and use P.I.D. controllers for precision and stability.

http://www.quorumtechnologies.com/Environment_Systems.html

Cheers,
Ryan Geil
------------------------------------------------------------
Quorum Technologies Inc.  
light at work
4673 Wellington Rd. #35 RR#6
Guelph, Ontario
Canada  N1H 6J3
www.quorumtechnologies.com

On 2011-04-05, at 7:35 PM, Leoncio Vergara wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi
>
> I was wondering if anybody could tell me about their experience with the Live-
> Cell stage top incubator from Pathology Devices Inc
> (http://www.pathologydevices.com/LiveCell.htm) or the Oko-lab Electric
> microscope stage incubator (http://www.oko-lab.com/39.page).
>
> Both seem to be very similar. We are looking for a solution for a BSL2 facility
> for live cell experiments on a Olympus DSU IX81 microscope with ZDCII. We
> want to do experiments with specifically with Labtek-type chambered
> coverglasses and 35 mm petri dishes (glass bottom).
>
> I am looking for quality and reliability for temperature and environmental
> control (CO2 and Humidity) as well as easy of use. For a multiuser facility like
> ours, simplicity and robust design are good. For BSL2, the use of disposable
> chambers is also desirable, even is we have to sacrifice some in terms of
> accuracy, etc.
>
> Thanks in advance for any information
>
> Leoncio Vergara
> UTMB, Galveston Texas

[Knecht, David]

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We have two Pathology Devices stage top incubators and they have worked very well for both chamber slides and Willco dishes.  I highly recommend it.  Dave

On Apr 5, 2011, at 7:35 PM, Leoncio Vergara wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi
>
> I was wondering if anybody could tell me about their experience with the Live-
> Cell stage top incubator from Pathology Devices Inc
> (http://www.pathologydevices.com/LiveCell.htm) or the Oko-lab Electric
> microscope stage incubator (http://www.oko-lab.com/39.page).
>
> Both seem to be very similar. We are looking for a solution for a BSL2 facility
> for live cell experiments on a Olympus DSU IX81 microscope with ZDCII. We
> want to do experiments with specifically with Labtek-type chambered
> coverglasses and 35 mm petri dishes (glass bottom).
>
> I am looking for quality and reliability for temperature and environmental
> control (CO2 and Humidity) as well as easy of use. For a multiuser facility like
> ours, simplicity and robust design are good. For BSL2, the use of disposable
> chambers is also desirable, even is we have to sacrifice some in terms of
> accuracy, etc.
>
> Thanks in advance for any information
>
> Leoncio Vergara
> UTMB, Galveston Texas

Dr. David Knecht    
Department of Molecular and Cell Biology
Co-head Flow Cytometry and Confocal Microscopy Facility
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)

[SUBSCRIBE CONFOCALMICROSCOPY jeroen van den akker]

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Hello Leoncio,

In response to your question: we bought the Oko-lab Electric incubator about
3 years ago. We ordered it with special inserts for 6- and 12-well plates, and
the chambered coverglass/35 mm dish insert as well. Oko-lab constructed
some special openings to enable fluid flow experiments (using Ibidi chambers).
The whole setup was really easy to install, and the system works stable for
multiple days without any adjustments. We also performed live-cell
fluorescence imaging (in a BSL2 lab), which worked fine in all insert types.

I hope this helps you.

Jeroen van den Akker
Academic Medical Center, Amsterdam/The Netherlands