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Dear list,
We are looking for a label that can allow us to judge the difference in amount of chromatin from 3D (widefield deconvolution or confocal) distributions of the label. Is there a stoichiometrically established probe for doing this? How about DAPI?
Our goal is to judge if some clones of colon cancer cells (fixed) that my friend has developed, have differing number of chromosomes without bothering to do karyotyping.
Any ideas/references are greatly appreciated.
best
shalin