systemic calcium dye application

[Stephan Speier]

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Dear List,

 

I wonder if anybody has experience with the systemic application of AM ester of calcium dyes like Fluo-4 or Fura-2 in living mice.

What other problems than the high costs per experiment would I have to expect?

I’m thinking of toxicity or similar.

 

Thanks for the help,

Stephan

 

[milton charlton]

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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

RE: systemic calcium dye application

This paper shows acumulation of Calcium crimson in hippocampus and  
cortex of rats injected with Calcium crimson-AM .  There are also  
electrophysiological recordings from brain slices of injected rats.


Tymianski M, Wallace MC, Spigelman I, Uno M, Carlen PL, Tator CH,  
Charlton MP. Cell-permeant Ca2+ chelators reduce early excitotoxic and  
ischemic neuronal
injury in vitro and in vivo. Neuron. 1993 Aug;11(2):221-35.


Milton P. Charlton, Professor
Physiology Department, University of Toronto
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