temperature contoler for stages

["José A. Feijó"]

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We need to cool and stabilize temperature to about 18-20oC for a live
cell experiment on room that cannot be brought bellow 22. Since we don't
need long sequences (a few minutes will do) as a cheap'o I was pondering
one of the these cooled stages, does any anyone has positive experience
with one of those? In the past we tried a Tokai, but it was not a happy
experience.

Thanks in advance

--
José A. Feijó, Professor
University of Maryland
Cell Biology and Molecular Genetics
0118 Bioscience Research Building
4066 Campus Dr.
College Park, MD 20742-5815
Phone [phone]
Fax [phone]
Email: [hidden email]

[Craig Brideau]

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If you will always be cooling (that is stage always < room temp) then you
only need a uni-directional controller. A cheap unit like an Omega
industrial controller (~$200) can run a small thermoelectric cooler with a
heat sink to dissipate the heat. If you really only need to drop it by a
few degrees then passive heat sinking on the TEC should be enough, assuming
the heat sink is large enough.
Otherwise, do you have access to chilled water? A trickle of that though
some tubing glued to a stage might work for you too, given that your delta
T is so small.

Craig

On Fri, Jun 17, 2016 at 12:55 PM, José A. Feijó <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> We need to cool and stabilize temperature to about 18-20oC for a live cell
> experiment on room that cannot be brought bellow 22. Since we don't need
> long sequences (a few minutes will do) as a cheap'o I was pondering one of
> the these cooled stages, does any anyone has positive experience with one
> of those? In the past we tried a Tokai, but it was not a happy experience.
>
> Thanks in advance
>
> --
> José A. Feijó, Professor
> University of Maryland
> Cell Biology and Molecular Genetics
> 0118 Bioscience Research Building
> 4066 Campus Dr.
> College Park, MD 20742-5815
> Phone [phone]
> Fax [phone]
> Email: [hidden email]
>

[Dan Focht]

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Jose


Although most of our customers are working at mammalian temperatures, Bioptechs has a variety of below ambient temperature regulation products.
How they are used is dependent on your scope configuration and experimental protocol.
Give us a call for details as our website does not feature many products for below ambient applications.


Dan

> On Jun 17, 2016, at 2:55 PM, José A. Feijó <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> We need to cool and stabilize temperature to about 18-20oC for a live cell experiment on room that cannot be brought bellow 22. Since we don't need long sequences (a few minutes will do) as a cheap'o I was pondering one of the these cooled stages, does any anyone has positive experience with one of those? In the past we tried a Tokai, but it was not a happy experience.
>
> Thanks in advance
>
> --
> José A. Feijó, Professor
> University of Maryland
> Cell Biology and Molecular Genetics
> 0118 Bioscience Research Building
> 4066 Campus Dr.
> College Park, MD 20742-5815
> Phone [phone]
> Fax [phone]
> Email: [hidden email]

Dan Focht
Bioptechs, Inc.
3560 Beck Rd.
Butler PA 16002
V724-282-7145
F724-282-0745
Toll Free 877 lIVE-CELL (548-3235)
[hidden email]

[Smith, Benjamin E.]

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We have a Physitemp TS-4MP that has worked extremely well.  It can control the temperature of both slides and 35mm culture dishes and in our experience it has an effective range of 0oC to 60oC and an accuracy of <0.1oC.  Not only do we use this stage for tissue culture (37oC) and lamprey embryos (18oC), but we've also used it in drosophila live imaging to keep the stage at exactly 25oC (which requires warming to start with, but then cooling once the microscope electronics warm up).  We've had a thermocouple in the culture dish and found that the dish stayed at exactly the set temperature within 0.1oC (the accuracy of our thermocouple) over 48 hours.  The PID controller also comes pre-tuned, so the stage settles at the set temperature within a minute.

We have a gentle flow of tap water over the backside of the Peltier device to ensure a constant thermal gradient across the device.

Hope this helps,

   Ben Smith



________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of Craig Brideau <[hidden email]>
Sent: Friday, June 17, 2016 3:29:40 PM
To: [hidden email]
Subject: Re: temperature contoler for stages

*****
To join, leave or search the confocal microscopy listserv, go to:
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*****

If you will always be cooling (that is stage always < room temp) then you
only need a uni-directional controller. A cheap unit like an Omega
industrial controller (~$200) can run a small thermoelectric cooler with a
heat sink to dissipate the heat. If you really only need to drop it by a
few degrees then passive heat sinking on the TEC should be enough, assuming
the heat sink is large enough.
Otherwise, do you have access to chilled water? A trickle of that though
some tubing glued to a stage might work for you too, given that your delta
T is so small.

Craig

On Fri, Jun 17, 2016 at 12:55 PM, José A. Feijó <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> We need to cool and stabilize temperature to about 18-20oC for a live cell
> experiment on room that cannot be brought bellow 22. Since we don't need
> long sequences (a few minutes will do) as a cheap'o I was pondering one of
> the these cooled stages, does any anyone has positive experience with one
> of those? In the past we tried a Tokai, but it was not a happy experience.
>
> Thanks in advance
>
> --
> José A. Feijó, Professor
> University of Maryland
> Cell Biology and Molecular Genetics
> 0118 Bioscience Research Building
> 4066 Campus Dr.
> College Park, MD 20742-5815
> Phone [phone]
> Fax [phone]
> Email: [hidden email]
>