Re: Perfect Focus

Posted by Mayandi Sivaguru on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Perfect-Focus-tp1098084p1098249.html

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Scott, it looks like you have a fully automated system. Personally, I do not rely on autofocus, I would suggest, if you take multi position, multi channels over multiple time point, then instead of one frame position/well also include a small z stack, say 4 images (2 above and 2 below) from the best focus of each position, so latter even if there is a drift, you will have an option to select the best plane out of those four.
Shiv

At 07:56 AM 9/18/2008, you wrote:

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

All,

Have new faculty coming aboard and a major application will be 48 hour
timelapse studies in 96 well plates using multichannel collections.
Therefore my question is how critical is it to have a system that has
a perfect focus mechanism to meet this application? Are there decent
work arounds if the perfect focus is not used? Have never actually
worked with this type of system so would like input on this matter
from the list. Thanks. 


Scott J. Howell, Ph.D.
Manager, Imaging Module
Visual Sciences Research Center
Case Western Reserve University
2085 Adelbert Rd.
Institute of Pathology Room 106
Cleveland, Ohio 44106
216-368-2300
http://www.case.edu/med/vsrc/

Mayandi Sivaguru, PhD, PhD
Microscopy Facility Manager
8, Institute for Genomic Biology
University of Illinois at Urbana-Champaign
1206 West Gregory Dr.
Urbana, IL 61801 USA

Office: 217.333.1214
Fax: [phone]
[hidden email]
http://core.igb.uiuc.edu