Re: Perfect Focus

Posted by Petrak, Lara J. on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Perfect-Focus-tp1098084p1098319.html

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Hi Scott,

I agree with Gabor and Simon that having some sort of "auto focusing"
mechanism (whether it be a z-motor based software autofocus or a
laser-based perfect focus system) is extremely helpful, and sometimes
essential, when doing long-term timelapse imaging.  I find that even
with temperature and humidity control, z-drift is likely over a long
period of time and needs to be corrected for.  If you plan to image
fluorescence with live cells over time, imaging multiple planes at each
time point is not ideal because of the burdens of increased
photobleaching and phototoxicity.

I have used both software autofocus, which tends to work just fine, and
the Nikon Perfect Focus System on the TE2000E.  The PFS is easy to use
and works very well (and much quickly than traditional autofocus!).  If
you look into this, make sure that the objective lenses that you plan to
use are rated to work with the PFS (I think that many will work, but
there are some exceptions).

Best,
Lara

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Lara Petrak
Microscopy Coordinator
 
Nikon Imaging Center @ Harvard Medical School
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