Re: Perfect Focus
Posted by
Ian Dobbie on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Perfect-Focus-tp1098084p1100441.html
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocalChris Tully <
[hidden email]> writes:
> well plates perfect focus systems can be VERY useful. While taking a z stack
> can help, I have seen more than one multi well plate with enough variation
> from well to well (especially from one end of the plate to the other) to
One important factor is to have a setup that allows position
definition in 3D, ie X,Y and Z. This saves problems from tilted, or
non-flat plates. I have done this with 72h time courses on 24 well
plates without any automatic focus adjustment, but with computer
control of x,y and z to visit the defined positions in 3D. With good
temperature stability this is not difficult at relatively low mag, say
10-20x. At high mag, or more importantly high NA, your depth of focus
is much smaller and therefore this is much more demanding.
Ian