fluorescent Ab counterstain for chicken somites (lectins?)
Posted by
matthewmakes on
URL: http://confocal-microscopy-list.275.s1.nabble.com/fluorescent-Ab-counterstain-for-chicken-somites-lectins-tp1130451.html
hi.
i'm interested in picking up a quick, simple and fairly affordable fluorescent counterstain for viewing cell morphology in antibody labeled sections of chicken embryos. from what i can put together, some sort of lectin would likely do the job, but i'm having a hard time finding material with lectin labels used in the chicken embryo. i've seen some papers with nice wheat germ agglutinin labels in similar staged somites of mice. i primarily need this as an Ab counterstain, but if i could get something that would work live as well, that would be great. (i've got a collection of membrane labels for live imaging, but more options are always nice.)
any advice would be greatly appreciated.
thank you, matthew
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