Re: fluorescent Ab counterstain for chicken somites (lectins?)

Posted by Carol Heckman on
URL: http://confocal-microscopy-list.275.s1.nabble.com/fluorescent-Ab-counterstain-for-chicken-somites-lectins-tp1130451p1134965.html

Matthew-
I don't seen any reason why the wheat germ agglutinin labels wouldn't work.  The cell membrane protein structure might be highly conserved between mouse and chicken.
Carol
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From: Confocal Microscopy List [[hidden email]] On Behalf Of Matthew Smith [[hidden email]]
Sent: Tuesday, September 30, 2008 9:12 PM
To: [hidden email]
Subject: fluorescent Ab counterstain for chicken somites (lectins?)

hi.

i'm interested in picking up a quick, simple and fairly affordable fluorescent counterstain for viewing cell morphology in antibody labeled sections of chicken embryos.  from what i can put together, some sort of lectin would likely do the job, but i'm having a hard time finding material with lectin labels used in the chicken embryo.  i've seen some papers with nice wheat germ agglutinin labels in similar staged somites of mice.  i primarily need this as an Ab counterstain, but if i could get something that would work live as well, that would be great.  (i've got a collection of membrane labels for live imaging, but more options are always nice.)

any advice would be greatly appreciated.

thank you,  matthew

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