cell membrane image

Posted by Koo, Lily (NIH/NIAID) [E] on
URL: http://confocal-microscopy-list.275.s1.nabble.com/fluorescent-Ab-counterstain-for-chicken-somites-lectins-tp1130451p1140188.html

Dear all,

When we imaging erythrocytes labeled with a membrane dye on a SP5
confocal, we noticed that in all images, the top and bottom of the cell
membrane appeared much dimmer than the left and right sides of it.  Is
there a particular reason for this observation?  Is there a difference
in the resolution of the x-scan vs. y-scan?

Thanks much.

Lily