Re: cell membrane image

Posted by Mark Cannell on
URL: http://confocal-microscopy-list.275.s1.nabble.com/fluorescent-Ab-counterstain-for-chicken-somites-lectins-tp1130451p1140214.html

Interesting observation. Assuming the PSF is not assymetric in x-y,
could this be due to the interaction of the orientation of the dye
molecules and the polarization of the laser?

Regards Mark Cannell


Koo, Lily (NIH/NIAID) [F] wrote:

> Dear all,
>
> When we imaging erythrocytes labeled with a membrane dye on a SP5
> confocal, we noticed that in all images, the top and bottom of the cell
> membrane appeared much dimmer than the left and right sides of it.  Is
> there a particular reason for this observation?  Is there a difference
> in the resolution of the x-scan vs. y-scan?
>
> Thanks much.
>
> Lily
>