Z sections

Posted by Matthew Pearson-3 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Z-sections-tp1142113.html


Hi all,

I have a question concerning Z sectioning.  We have a Leica SP2 and when set the top and bottom range for a Z stack, the software automatically calculates the optimised number of sections, which is nearly always over 100!  This is however not practical as the stacks take too long to acquire.  Can anyone shed some light on how the software decides how many slices are necessary and if I haven't got time to take 100, how can I work out what will be a sufficient number.

Thanks for your help in advance.

Matt Pearson.