Re: Z sections

Posted by Peter Carroll on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Z-sections-tp1142113p1142181.html

 > how the software decides how many slices are necessary and if I
haven't got time to take 100, how can I work out what will be a
sufficient number.

I guess it really depends on your goal, or what you're looking for, as
I'm not certain there's any arbitrary "sufficient number"...

So what are you looking for/at?




Matthew Pearson wrote:

>
> Hi all,
>
> I have a question concerning Z sectioning.  We have a Leica SP2 and
> when set the top and bottom range for a Z stack, the software
> automatically calculates the optimised number of sections, which is
> nearly always over 100!  This is however not practical as the stacks
> take too long to acquire.  Can anyone shed some light on how the
> software decides how many slices are necessary and if I haven't got
> time to take 100, how can I work out what will be a sufficient number.
>
> Thanks for your help in advance.
>
> Matt Pearson.
>
>
>