Re: Z sections

Posted by Csúcs Gábor on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Z-sections-tp1142113p1142215.html

Dear Matt,

Typically such calculations are done by calculating the maximal optical
resolution in Z-direction for the given objective (NA) and pinhole size
and taking a 2x oversampling. This is how it is done e.g. in the Zeiss
software. The Leica SP2 unfortunately doesn't do this automatically - as
far as I recall it takes the X-Y resolution values also for calculating
the Z-sections. You can kind of cheat the system by setting the
oversampling factor to 2. (Under Settings/Scan options/Sampling factor).

Cheers   Gabor

>
> Hi all,
>
> I have a question concerning Z sectioning.  We have a Leica SP2 and
> when set the top and bottom range for a Z stack, the software
> automatically calculates the optimised number of sections, which is
> nearly always over 100!  This is however not practical as the stacks
> take too long to acquire.  Can anyone shed some light on how the
> software decides how many slices are necessary and if I haven't got
> time to take 100, how can I work out what will be a sufficient number.
>
> Thanks for your help in advance.
>
> Matt Pearson.
>
>
>

--
Gabor Csucs
Light Microscopy Centre, ETH Zurich
Schafmattstrasse 18, HPM F16
CH-8093, Zurich, Switzerland

Web: www.lmc.ethz.ch
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