Re: fluorescent Ab counterstain for chicken somites (lectins?)

Posted by Boswell, Carl A - (cboswell) on
URL: http://confocal-microscopy-list.275.s1.nabble.com/fluorescent-Ab-counterstain-for-chicken-somites-lectins-tp1130451p1142659.html

Hi Matthew,
As a point of clarification, lectins will bind specific sequences of sugars
on glycoproteins.  As such, identical proteins with different glyco-residues
will have different lectin binding properties.  Given that, you may need to
do what I had to do years ago, and that was sample a bunch of different
lectins to see what works.  When  lectin chemistry was new, one could find
samplers with small amounts of several lectins to test.  Now, I don't see
any.  Sigma Chemical has many lectins, though I don't know how many have
been fluorescently tagged.  Molecular Probes is another source of labelled
lectins.
Good luck,
Carl

Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
University of Arizona
520-954-7053
FAX [phone]
----- Original Message -----
From: "Carol Heckman" <[hidden email]>
To: <[hidden email]>
Sent: Thursday, October 02, 2008 12:22 PM
Subject: Re: fluorescent Ab counterstain for chicken somites (lectins?)


Matthew-
I don't seen any reason why the wheat germ agglutinin labels wouldn't work.
The cell membrane protein structure might be highly conserved between mouse
and chicken.
Carol
________________________________________
From: Confocal Microscopy List [[hidden email]] On Behalf
Of Matthew Smith [[hidden email]]
Sent: Tuesday, September 30, 2008 9:12 PM
To: [hidden email]
Subject: fluorescent Ab counterstain for chicken somites (lectins?)

hi.

i'm interested in picking up a quick, simple and fairly affordable
fluorescent counterstain for viewing cell morphology in antibody labeled
sections of chicken embryos.  from what i can put together, some sort of
lectin would likely do the job, but i'm having a hard time finding material
with lectin labels used in the chicken embryo.  i've seen some papers with
nice wheat germ agglutinin labels in similar staged somites of mice.  i
primarily need this as an Ab counterstain, but if i could get something that
would work live as well, that would be great.  (i've got a collection of
membrane labels for live imaging, but more options are always nice.)

any advice would be greatly appreciated.

thank you,  matthew

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