Posted by
vb-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Re-Ana-alarming-amount-of-image-manipulation-tp592857p1316784.html
Hi Lily,
Yes, they will bind each other at the membrane. Adding L221K, F223R
mutations to the Cerulean and all three mutations to venusYFP may reduce
this hetero-dimerization (Cerulean has the A206K mutation, as published).
Depending on the protein and the level of overexpression (as many young
folks are in a "blind love" with a very strong CMV promoter and/or COS-7 or
293T cells which are transgenic for T-large antigen), CeFP-venusYFP
co-aggregation is a well known phenomena.
If you have any specific questions, please contact me off-line.
Vitaly
NCI-Frederick,
301-846-6575
----- Original Message -----
From: "Koo, Lily (NIH/NIAID) [F]" <
[hidden email]>
To: <
[hidden email]>
Sent: Friday, October 10, 2008 9:15 AM
Subject: Cerulean Venus binding possible?
> Hello all,
>
> One of my colleagues is conducting an experiment in which she studies
> the diffusion of her membrane protein tagged with Cerulean; also present
> in her cell is another membrane protein tagged with Venus. Her control
> experiments seem to suggest a possibility that Cerulean and Venus may
> bind to each other.
>
> Has anyone had similar observations or suspicion, at least in cell
> membranes?
>
> Thanks,
>
> Lily
>