Re: Cerulean Venus binding possible?

Posted by Kurt Thorn on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Re-Ana-alarming-amount-of-image-manipulation-tp592857p1317090.html

It's been well documented that GFP and its derivatives will dimerize.  
The Kd is about 100 uM; mutating A206 to K or R abolishes this dimerization.

See http://www.ncbi.nlm.nih.gov/pubmed/11988576 for the details.

Kurt

Koo, Lily (NIH/NIAID) [F] wrote:

> Hello all,
>
> One of my colleagues is conducting an experiment in which she studies
> the diffusion of her membrane protein tagged with Cerulean; also present
> in her cell is another membrane protein tagged with Venus.  Her control
> experiments seem to suggest a possibility that Cerulean and Venus may
> bind to each other.  
>
> Has anyone had similar observations or suspicion, at least in cell
> membranes?
>
> Thanks,
>
> Lily
>
>  

--
Kurt Thorn, PhD
Director, Nikon Imaging Center
University of California San Francisco

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