FRET on FV500

Posted by js1719 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/FRET-on-FV500-tp1337622.html

Hello,
  I have a user that is going to do FLIM-FRET on our Olympus FV500  
system soon and I was wondering if any other people on the list serve  
have an established (written) protocol for doing either de-
constructive acceptor photo-bleaching (using the protocol processor  
method) or synthesized emission. We added the FRET module a few years  
ago but no one here ever used it. He is set up to use CFP/YFP. I have  
told him to do CFP and YFP alone as controls and I think I will teach  
him the de-constructive method first since as I remember it being  
easier. I also wondered what analysis program people use for  
calculating FRET these days- we tend to use ImageJ a lot for analysis  
so if you have a plugin that works well can you let me know? I  
appreciate all the advice you can give me, this is user is completely  
new to microscopes so the more I can provide him with the better.
Best,
  Julia