Usefulness of 1040-1080 nm in 2P Excitation

Posted by Mayandi Sivaguru on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Usefulness-of-1040-1080-nm-in-2P-Excitation-tp1346615.html


Hi all, I have seen articles using 1030 nm Ex for mCherry, but the Coherent site showing data that it is best excited with 1060 nm, how significant the range 1040-1080 nm apart from mCherry in your experience for any other probes. In my personal experience, attempts to tune the laser closer to low and high end of the tuning range (730-930nm, particularly trying to obtain an excitation profile), in a previous version of Chameleon often tend to send the laser out of mode lock and requires a 15-30 min PZT recovery.

Thanking you in advance for your insights in this regard
Shiv

Mayandi Sivaguru, PhD, PhD
Microscopy Facility Manager
8, Institute for Genomic Biology
University of Illinois at Urbana-Champaign
1206 West Gregory Dr.
Urbana, IL 61801 USA

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