Re: deep UV imaging

Posted by Mario-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/deep-UV-imaging-tp1364588p1364929.html

Quartz optics or at least objectives (does anybody make these,
currently?) and coverslips (fragile and expensive).

Once tried three photon excitation with some success. Might be a
reasonable option.

Mario

>Hi everyone,
>
>I was wondering if any of you has any experience with deep UV
>fluorescence imaging? In particular I would be interested in looking
>at the fluorescence of tryptophan (excitation around 280 nm,
>emission above 350 nm). Which modifications would be necessary of a
>standard fluorescence microscope for imaging in this region?
>
>Thanks for any thoughts/suggestions on this.
>
>Kevin
>
>
>
>Prof. Dr. Kevin Braeckmans
>Lab. General Biochemistry and Physical Pharmacy
>Ghent University
>Harelbekestraat 72
>9000 Ghent
>Belgium
>Tel: [phone]
>Fax: [phone]
>
>
>
>

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________________________________________________________________________________
Mario M. Moronne, Ph.D.

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