Re: deep UV imaging

Posted by Armstrong, Brian on
URL: http://confocal-microscopy-list.275.s1.nabble.com/deep-UV-imaging-tp1364588p1364954.html

Hello, I believe that Leica and Zeiss make quartz optics for use in
their industrial instruments used for Mask Alignment. These optics are
incredibly expensive because of the quartz and because adhesives cannot
be used in their construction due to the corrosive nature of UV. I know
that researchers in protein crystallography are interested in crystal
illumination in UV and that some have built their own scopes with quartz
optics. I think Reichert-Jung made quartz optics for their industrial
scopes in the past as well. Something to keep in mind if you build your
own scope is SAFETY with regards to sending UV light to the eyepieces.
Cheers,

Brian D Armstrong PhD
Light Microscopy Core Manager
Beckman Research Institute
City of Hope
Dept of Neuroscience
1450 E Duarte Rd
Duarte, CA 91010
626-256-4673 x62872
http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imag
ing/Pages/default.aspx
-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Mario
Sent: Wednesday, October 22, 2008 10:29 AM
To: [hidden email]
Subject: Re: deep UV imaging

Quartz optics or at least objectives (does anybody make these,
currently?) and coverslips (fragile and expensive).

Once tried three photon excitation with some success. Might be a
reasonable option.

Mario

>Hi everyone,
>
>I was wondering if any of you has any experience with deep UV
>fluorescence imaging? In particular I would be interested in looking
>at the fluorescence of tryptophan (excitation around 280 nm,
>emission above 350 nm). Which modifications would be necessary of a
>standard fluorescence microscope for imaging in this region?
>
>Thanks for any thoughts/suggestions on this.
>
>Kevin
>
>
>
>Prof. Dr. Kevin Braeckmans
>Lab. General Biochemistry and Physical Pharmacy
>Ghent University
>Harelbekestraat 72
>9000 Ghent
>Belgium
>Tel: [phone]
>Fax: [phone]
>
>
>
>

--
________________________________________________________________________
________
Mario M. Moronne, Ph.D.

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[hidden email]
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