Re: deep UV imaging

Posted by Kurt Thorn on
URL: http://confocal-microscopy-list.275.s1.nabble.com/deep-UV-imaging-tp1364588p1365161.html

There was a recent paper in Nature Methods that built a UV
transillumination microscope for imaging protein absorption:
http://www.nature.com/nmeth/journal/v4/n7/full/nmeth1053.html

Kurt

Kevin Braeckmans wrote:

>
> Hi everyone,
>
>  
>
> I was wondering if any of you has any experience with deep UV
> fluorescence imaging? In particular I would be interested in looking
> at the fluorescence of tryptophan (excitation around 280 nm, emission
> above 350 nm). Which modifications would be necessary of a standard
> fluorescence microscope for imaging in this region?
>
>  
>
> Thanks for any thoughts/suggestions on this.
>
>  
>
> Kevin
>
>  
>
>  
>
>  
>
> Prof. Dr. Kevin Braeckmans
>
> Lab. General Biochemistry and Physical Pharmacy
>
> Ghent University
>
> Harelbekestraat 72
>
> 9000 Ghent
>
> Belgium
>
> Tel: [phone]
>
> Fax: [phone]
>
>  
>
>  
>
>  
>
>  
>

--
Kurt Thorn, PhD
Director, Nikon Imaging Center
University of California San Francisco

UCSF MC 2140
Genentech Hall Room S252
600 16th St.
San Francisco, CA 94158-2517

http://nic.ucsf.edu
phone [phone]
fax   [phone]