Confocal Microscopy List

Re: deep UV imaging

Posted by Kevin Braeckmans on
URL: http://confocal-microscopy-list.275.s1.nabble.com/deep-UV-imaging-tp1364588p1381313.html

Thanks to all who have responded, much appreciated!

I will go in more detail through the articles and other information that was suggested. For now I will keep in mind that it is not impossible, but that it will require a dedicated set-up (and budget). Triple-photon imaging is not an option in our case since the project would involve fast widefield imaging (>50 fps).

Thanks again and best regards,

Kevin

Van: Confocal Microscopy List [mailto:[hidden email]] Namens Turan Erdogan
Verzonden: woensdag 22 oktober 2008 21:16
Aan: [hidden email]
Onderwerp: Re: deep UV imaging

Kevin:

Two things. First, there is a company called CRAIC (no personal affiliation) that reconfigures standard microscope stands into UV-compatible microscopes. See, for example: http://www.microspectra.com/products/uvm-1-vis-nir-microscope/34-products/53-uvm-1-ultraviolet-microscope?56563afcc766aeae8c9d5b9b84d9674a=8cba804d502960fbac4d5fa507955c58. These are also capable of fluorescence imaging. The microscope uses UV optics (objective as well as excitation-beam-line optics and tube lens).

An additional challenge is the filters. Semrock (I AM commercially affiliated with Semrock) sells a filter set specifically for imaging tryptophan (see http://www.semrock.com/Catalog/Brightline_TRP.htm).

Hope that's helpful.

-Turan

Dr. Turan Erdogan, CTO

Semrock, Inc.

3625 Buffalo Road

Rochester, NY 14624

tel: (585) 594-7001

fax: (585) 594-7095

[hidden email]

www.semrock.com

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Kevin Braeckmans
Sent: Wednesday, October 22, 2008 12:05 PM
To: [hidden email]
Subject: deep UV imaging

Hi everyone,

I was wondering if any of you has any experience with deep UV fluorescence imaging? In particular I would be interested in looking at the fluorescence of tryptophan (excitation around 280 nm, emission above 350 nm). Which modifications would be necessary of a standard fluorescence microscope for imaging in this region?

Thanks for any thoughts/suggestions on this.

Kevin

Prof. Dr. Kevin Braeckmans

Lab. General Biochemistry and Physical Pharmacy

Ghent University

Harelbekestraat 72

9000 Ghent

Belgium

Tel: +32 (0)9 264.80.78

Fax: +32 (0)9 264.81.89