Re: deep UV imaging

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/deep-UV-imaging-tp1364588p1383242.html

Don't know if this is useful but Thorlabs sells UV objectives:

http://www.thorlabs.com/NewGroupPage9.cfm?ObjectGroup_ID=3271

No commercial interest, just use a lot of Thor stuff.

Craig

On Mon, Oct 27, 2008 at 1:11 AM, Kevin Braeckmans <[hidden email]> wrote:

Thanks to all who have responded, much appreciated!

 

I will go in more detail through the articles and other information that was suggested. For now I will keep in mind that it is not impossible, but that it will require a dedicated set-up (and budget). Triple-photon imaging is not an option in our case since the project would involve fast widefield imaging (>50 fps).

 

Thanks again and best regards,

 

Kevin

 

Van: Confocal Microscopy List [mailto:[hidden email]] Namens Turan Erdogan
Verzonden: woensdag 22 oktober 2008 21:16
Aan: [hidden email]
Onderwerp: Re: deep UV imaging

 

Kevin:

 

Two things.  First, there is a company called CRAIC (no personal affiliation) that reconfigures standard microscope stands into UV-compatible microscopes.  See, for example:  http://www.microspectra.com/products/uvm-1-vis-nir-microscope/34-products/53-uvm-1-ultraviolet-microscope?56563afcc766aeae8c9d5b9b84d9674a=8cba804d502960fbac4d5fa507955c58.  These are also capable of fluorescence imaging.  The microscope uses UV optics (objective as well as excitation-beam-line optics and tube lens).

 

An additional challenge is the filters.  Semrock (I AM commercially affiliated with Semrock) sells a filter set specifically for imaging tryptophan (see http://www.semrock.com/Catalog/Brightline_TRP.htm).

 

Hope that's helpful.

 

-Turan

 

Dr. Turan Erdogan, CTO

Semrock, Inc.

3625 Buffalo Road

Rochester, NY 14624

tel:  [phone]

fax:  [phone]

[hidden email]

www.semrock.com

 

 

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From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Kevin Braeckmans
Sent: Wednesday, October 22, 2008 12:05 PM
To: [hidden email]
Subject: deep UV imaging

Hi everyone,

 

I was wondering if any of you has any experience with deep UV fluorescence imaging? In particular I would be interested in looking at the fluorescence of tryptophan (excitation around 280 nm, emission above 350 nm). Which modifications would be necessary of a standard fluorescence microscope for imaging in this region?

 

Thanks for any thoughts/suggestions on this.

 

Kevin

 

 

 

Prof. Dr. Kevin Braeckmans

Lab. General Biochemistry and Physical Pharmacy

Ghent University

Harelbekestraat 72

9000 Ghent

Belgium

Tel: [phone]

Fax: [phone]