Re: Alexa 750

Posted by G. Esteban Fernandez on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Alexa-750-tp1459204p1462483.html

AF750 has been tried in my lab on a 510 META with no success (633 excitation).  As has been mentioned by others, the standard laser lines and emission filters are not suitable.  We ended up going on a widefield 'scope with an AF750 filter from Chroma and a metal halide lamp.  Even though the output of metal halide and the QE of the camera are both poor at ≥750 nm, we got good images with long integration times (> 1 sec.) on a cooled CCD camera.

 

-Esteban

On Wed, Nov 5, 2008 at 2:32 AM, Olivier Bardot <[hidden email]> wrote:

Hello
We are using DAPI, FITC, Cy3 and Cy5 for immunolabelling. We want to use a
fifth label and have found that Alexa750 seems to be a good possibility.
We are working on Zeiss LSM 510 Meta.
Does anyone have been using this dye?
Is it really possible to discriminate Cy3 from Alexa750?
I will appreciate any advices.
Thanks

--
G. Esteban Fernandez, Ph.D.
Associate Director
Molecular Cytology Core Facility
University of Missouri
120 Bond Life Sciences Center
Columbia, MO  65211

http://www.biotech.missouri.edu/mcc/

573-882-4895
573-884-9395 fax