Posted by
M. van de corput on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Alexa-750-tp1459204p1466566.html
Another option is using combinatorial labeling. I don't know if that is
possible for your targets but it will avoid an increase in scan time and
you can use the labels you have used before and are familiar with.
Mariette
____________________________________________
Dr. M.P.C. Kemner-van de Corput,
____________________________________________
MGC - Dept. of Cell Biology & Genetics
Erasmus Medical Center
Dr. Molewaterplein 50, 3015 GE Rotterdam
POB 2040, 3000 CA Rotterdam, The Netherlands
Office: H-Ee751; tel: +31 10 704.3949
Lab: H-Ee710; tel lab: +31 10 704.3315
tel secr: +31 10 704.3169
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http://www2.eur.nl/fgg/ch1/cellbiology/http://www.thesis.kemner.biz/____________________________________________
Op Wo, 5 november, 2008 11:58 pm, schreef G. Esteban Fernandez:
> AF750 has been tried in my lab on a 510 META with no success (633
> excitation). As has been mentioned by others, the standard laser lines
> and
> emission filters are not suitable. We ended up going on a widefield
> 'scope
> with an AF750 filter from Chroma and a metal halide lamp. Even though the
> output of metal halide and the QE of the camera are both poor at 750 nm,
> we
> got good images with long integration times (> 1 sec.) on a cooled CCD
> camera.
>
> -Esteban
>
> On Wed, Nov 5, 2008 at 2:32 AM, Olivier Bardot <
[hidden email]> wrote:
>
>> Hello
>> We are using DAPI, FITC, Cy3 and Cy5 for immunolabelling. We want to use
>> a
>> fifth label and have found that Alexa750 seems to be a good possibility.
>> We are working on Zeiss LSM 510 Meta.
>> Does anyone have been using this dye?
>> Is it really possible to discriminate Cy3 from Alexa750?
>> I will appreciate any advices.
>> Thanks
>>
>
>
>
> --
> G. Esteban Fernandez, Ph.D.
> Associate Director
> Molecular Cytology Core Facility
> University of Missouri
> 120 Bond Life Sciences Center
> Columbia, MO 65211
>
>
http://www.biotech.missouri.edu/mcc/>
> 573-882-4895
> 573-884-9395 fax
>