Re: Alexa 750 - Vendor Response
Posted by
Kilgore, Jason on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Alexa-750-tp1459204p1466919.html
**Vendor Response**
Hi, all,
I should note that we have a 510 META here at Molecular
Probes. Though I don't think I have tried using Alexa Fluor 750 on it,
Qdot 800 is not able to be visualized on that system. Qdot 705, or
anything lower, is able to be imaged, though.
Of course, widefield microscopes work fine with Alexa Fluor
750 or Qdot 800 (with correct filter sets and detectors).
Cheers,
Jason
Jason A. Kilgore
Invitrogen Corporation
Molecular Probes Labeling and Detection
Technologies
Technical Service Scientist
Eugene, OR 97402-9132
phone: (541) 335-0353
fax: (541) 335-0238
P Save trees. Please don't print
this e-mail unless you really need to.
AF750 has been tried in my lab on a 510 META with no success (633
excitation). As has been mentioned by others, the standard laser lines and
emission filters are not suitable. We ended up going on a widefield 'scope
with an AF750 filter from Chroma and a metal halide lamp. Even though the
output of metal halide and the QE of the camera are both poor at ≥750 nm,
we got good images with long integration times (> 1 sec.) on a cooled CCD
camera.
-Esteban
On Wed, Nov 5, 2008 at 2:32 AM, Olivier Bardot
<[hidden email]>
wrote:
Hello
We
are using DAPI, FITC, Cy3 and Cy5 for immunolabelling. We want to use
a
fifth label and have found that Alexa750 seems to be a good
possibility.
We are working on Zeiss LSM 510 Meta.
Does anyone have been
using this dye?
Is it really possible to discriminate Cy3 from
Alexa750?
I will appreciate any
advices.
Thanks
--
G. Esteban
Fernandez, Ph.D.
Associate Director
Molecular Cytology Core
Facility
University of Missouri
120 Bond Life Sciences Center
Columbia,
MO 65211
http://www.biotech.missouri.edu/mcc/573-882-4895
573-884-9395
fax