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GFP bleaching

Posted by M. Jarnik on Nov 10, 2008; 2:36pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/GFP-bleaching-tp1480738.html

Hi Listers,

I am trying some FRAP experiments on our Nikon C1 confocal. In particular, I would neet to
bleach GFP on microtubules in mitotic cells. While bleaching in mounted fixed samples
seems to work well, we have problems in life spls (using a heated chamber). Any magic
touch we might be missing?

Thanks,

Michal