Re: GFP bleaching

Posted by Alberto Diaspro on
URL: http://confocal-microscopy-list.275.s1.nabble.com/GFP-bleaching-tp1480738p1480749.html

Temperature increase makes photobleaching faster..
Il giorno 10/nov/08, alle ore 15:36, M. Jarnik ha scritto:

> Hi Listers,
>
> I am trying some FRAP experiments on our Nikon C1 confocal. In  
> particular, I would neet to
> bleach GFP on microtubules in mitotic cells. While bleaching in  
> mounted fixed samples
> seems to work well, we have problems in life spls (using a heated  
> chamber). Any magic
> touch we might be missing?
>
> Thanks,
>
> Michal

-----------------------------------------------------
Resistere, Resistere, Resistere!  Hold out, Hold out, Hold out!
-----------------------------------------------------
Alberto Diaspro
Department of Physics, University of Genoa,
Via Dodecaneso 33, 16146 Genoa, Italy
fax [phone] - tel [phone];
email: [hidden email] - URL: www.lambs.it;

THINK EBSA2009! www.ebsa2009.org
  ----------------------------------------------