Posted by
Xuejun Sun on
URL: http://confocal-microscopy-list.275.s1.nabble.com/GFP-bleaching-tp1480738p1481791.html
Your time resolution is maybe too low either recovering happened or sample
moved... Here are few options: zoom up/increase laser power and reduce
number of bleaching cycles, reduce image area and/or use higher frame rate.
Good luck!
Xuejun
Xue-jun Sun, Ph.D.
Dept. Exp. Oncology
Cross Cancer Institute
11560 University Ave,
Edmonton Alberta T6G 1Z2
Canada
Phone: (780) 432-8898
Fax: (780) 432-8425
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On
Behalf Of M. Jarnik
Sent: Monday, November 10, 2008 7:36 AM
To:
[hidden email]
Subject: GFP bleaching
Hi Listers,
I am trying some FRAP experiments on our Nikon C1 confocal. In particular, I
would neet to
bleach GFP on microtubules in mitotic cells. While bleaching in mounted
fixed samples
seems to work well, we have problems in life spls (using a heated chamber).
Any magic
touch we might be missing?
Thanks,
Michal
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