Re: Practice samples

Posted by Mollie Lange on
URL: http://confocal-microscopy-list.275.s1.nabble.com/ATTO647N-and-FCS-tp1499210p1499626.html

Jonathan,

 

You can order all kinds of prepared fluorescent slides from Molecular Probes/Invitrogen.

>>> Jonathan M Krupp <[hidden email]> 11/14/2008 11:45 AM >>>
Hi:

This is a pretty basic and simple question. I need some
advice about rounding up some samples to use in an
elementary light microscopy class that includes basic
fluorescence.

I am at a community college and my background is in EM and
brightfield LM. I have a passing familiarity with confocal
et al but not enough to know much. I maintained a
confocal, but never ran experiments or prepared much in
the way of samples. Now I have to get something together
to demonstrate the fundamentals of fluorescence to
students in a new job.

I have been relying on chlorophyll autofluorescence up til
now, but would like to add anything that would be easy to
do. We have a simple scope with filters for FITC,
rhodamine, and DAPI, I think. What would be some fool
proof, easy to get samples to try?

In addition, I would eventually like to add some kind of
confocal experience to this class, any ideas about where
to find an inexpensive system would be great.

Thanks

Jon
San Joaquin Delta College
Stockton, CA 95207
[hidden email]

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