http://confocal-microscopy-list.275.s1.nabble.com/ATTO647N-and-FCS-tp1499210p1499913.html
> Jon,
>
> I recommend brine shrimp 3 days old or older, fixed with formalin
> and stained with rhodamine-phalloidin, which shows up the muscles
> nicely. I use this as the first sample to look at in my confocal
> microscopy class. Brine shrimp are always available - just add
> water. The biggest up front cost in the rhodamine-phalloidin, but
> you can keep it for years.
>
> Phil Hertzler
>
> At 11:45 AM 11/14/2008, you wrote:
>> Hi:
>>
>> This is a pretty basic and simple question. I need some advice
>> about rounding up some samples to use in an elementary light
>> microscopy class that includes basic fluorescence.
>>
>> I am at a community college and my background is in EM and
>> brightfield LM. I have a passing familiarity with confocal et al
>> but not enough to know much. I maintained a confocal, but never ran
>> experiments or prepared much in the way of samples. Now I have to
>> get something together to demonstrate the fundamentals of
>> fluorescence to students in a new job.
>>
>> I have been relying on chlorophyll autofluorescence up til now, but
>> would like to add anything that would be easy to do. We have a
>> simple scope with filters for FITC, rhodamine, and DAPI, I think.
>> What would be some fool proof, easy to get samples to try?
>>
>> In addition, I would eventually like to add some kind of confocal
>> experience to this class, any ideas about where to find an
>> inexpensive system would be great.
>>
>> Thanks
>>
>> Jon
>> San Joaquin Delta College
>> Stockton, CA 95207
>>
[hidden email]
>
> Philip L. Hertzler
> Associate Professor
> Central Michigan University
> Dept. of Biology, Brooks Hall 217
> Mount Pleasant, MI 48859
>
> Phone: (989) 774-2393
> Fax: (989) 774-3462
> Email:
[hidden email]
>
> "Teachers should be paid more."
> - Sarah Palin, Vice-presidential debate, 10/2/2008
>
> "It is common sense to take a method and try it. If it fails, admit
> it frankly and try another. But above all, try something."
> - Franklin D. Roosevelt
>
> "Not everything that counts can be counted, and not everything that
> can be counted counts."
> - attributed to Albert Einstein