Posted by
Glen MacDonald-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Laser-effects-during-bleaching-tp1515193p1520447.html
Many aquatic invertebrates have autofluorescence spanning the spectrum
from UV to red. Also, a DAB immunolabeled tissue slide dipped briefly
in glutaraldehyde and rinsed before coverslipping will display green-
yellow fluorescence with dark DAB deposits.
Glen
On Nov 19, 2008, at 11:41 AM, Jonathan M Krupp wrote:
> On Tue, 18 Nov 2008 19:46:15 -0700
> John Chandler <
[hidden email]> wrote:
>> So, Jon,
>> Which demo samples do you like? Which ones have good effort to
>> results ratio?
>> Inquiring minds want to know.
>
>
> I'll get a more comprehensive list together and send it to you, but
> for now here's what I have tried.
>
> Simple H&E sections are amazingly useful. I didn't believe it when
> someone suggested it, but they really work to demo fluorescence in a
> simple way.
>
> I am going to try pollen and some freehand sections of some local
> plants asap. Also looks like the prepared test slides from
> Invitrogen might be a good bet if you can afford them.
>
> Jon