Re: Laser effects during bleaching

Posted by Csúcs Gábor on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Laser-effects-during-bleaching-tp1515193p1556349.html

Dear Stephen,

I'd argue that photobleaching is a procedure which we barely understand.
Most of the observations/rules are purely empirical and so far I have
seen no comprehensive description of the procedure. Furthermore (as
always) the details may be dependent also on the specific dye/fluorophore.
But back to your question: of course the reactive species created in a
photobleaching experiment will harm also other proteins (or generally
the cell). However, the overall assumption is that your
bleaching/recovery experiment happens in a much faster time scale then
the corresponding cytotoxic effects. Of course there are
processes/methods like CALI (Chromophore Assisted Laser Inactivation)
where people used the "immediate" effect of the putative reactive
species (as far as I know also CALI is a poorly understood procedure).

Greetings    Gabor

--
Gabor Csucs
Light Microscopy Centre, ETH Zurich
Schafmattstrasse 18, HPM F16
CH-8093, Zurich, Switzerland

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