Re: GFP and lipid localization?

Posted by Mario-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Laser-effects-during-bleaching-tp1515193p1558767.html

I have the same question, and, further, are you sure that the GFP
isn't simply aggregated? If aggregates form they could expose
hydrophobic surfaces that favorably bind to the droplets.

Mario

>Hi David - are you certain those are really lipid droplets and not
>something like endocytic vesicles?
>
>Mike Model
>
>-----Original Message-----
>From: Confocal Microscopy List
>[mailto:[hidden email]] On Behalf Of David Burk
>Sent: Thursday, November 20, 2008 2:31 PM
>To: [hidden email]
>Subject: GFP and lipid localization?
>
>GFP Experts:
>
>We have a group transfecting cells with adenovirus.  This virus contains
>an EGFP sequence to let you know that, yes, the cell was transfected.
>We have noticed in some of the transfected cells that the GFP signal
>appears to be strongly associated with lipid droplets.  This is new to
>me as I wouldn't expect WT-GFP to strongly associate with any particular
>subcellular organelle and is confounding their work since they are
>primarily interested in - of course - lipid droplets.
>
>Do any of you know of a reason we would see wild-type GFP with no
>targeting sequence or modifications labeling lipid droplets?  We are
>beginning some additional control experiments with a large titration
>range of virus to determine if the lipid labeling is related to vector
>concentration (~GFP expression level).
>
>Thanks for your help!
>
>David H. Burk

--
________________________________________________________________________________
Mario M. Moronne, Ph.D.

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