I would like to thank everyone who replied to the
Deconvolution Algorithm questions. Once I Get back to the lab on Monday we will
start comparing the suggested algorithms. Thanks Brian for your comments as
well, the commercial product we are comparing against is the Autoquant module
in the Nikon NIS elements software.
I do have a question for all of you regarding calculating
the point spread function. For some of the open source algorithms we are
using we have to input the PSF in the deconvolution algorithm. For this
we using a an open source software which calculates theoretical PSF after you
input imaging parameters such as, magnification, NA etc. but this software does
not have Laser Scanning Confocal microscopy as an imaging modality option, it
only has wide field fluorescence. Is there a formula for theoretically calculating
PSF in which you can input the Confocal pin hole as a parameter, or would
it be easier to determine the PSF experimentally using fluorescent beads?
Best Regards,
Neeraj.
Neeraj
V. Gohad, PhD
Postdoctoral
Fellow,
Okeanos
Research Group
Department
of Biological Sciences
132 Long
Hall,Clemson University
Clemson,
SC-29634
864-656-3597
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