Removing slit-scanning confocal line artifacts

Posted by G. Esteban Fernandez on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Removing-slit-scanning-confocal-line-artifacts-tp1635395.html

Hello all,

 

We sometimes see vertical line artifacts in images acquired with our slit-scanning confocal.  The pattern is highly reproducible and can be divided out with a "blank" image.  However, we sometimes have to filter out the artifacts in Fourier space by masking with a thin horz. rectangle through the middle of the FFT map, save the very center.  Can someone please point out references where line artifacts have been removed with a filter (not by dividing out), whether in Fourier space or not?

 

Those interested can see sample images and FFT mask at http://www.mediafire.com/?sharekey=7b16b66ca9129ce991b20cc0d07ba4d2a83703a20834b252

 

Thanks,

Esteban

--
G. Esteban Fernandez, Ph.D.
Associate Director
Molecular Cytology Core Facility
University of Missouri
120 Bond Life Sciences Center
Columbia, MO  65211

http://www.biotech.missouri.edu/mcc/

573-882-4895
573-884-9395 fax