Re: Removing slit-scanning confocal line artifacts

Posted by Zoltan on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Removing-slit-scanning-confocal-line-artifacts-tp1635395p1635946.html

Hi Esteban,

I can't give you a reference, unfortunately, but I can confirm that we had the same problem years ago with a Zeiss LSM5 live; I wrote an automated pre-processing tool in IDL that removed the critical part from the FFT and did the inverse FFT for all images.  The problem was eventually solved by Zeiss.

  Hope this helps,

Zoltan

On Tue, Dec 9, 2008 at 7:25 PM, G. Esteban Fernandez <[hidden email]> wrote:

Hello all,

 

We sometimes see vertical line artifacts in images acquired with our slit-scanning confocal.  The pattern is highly reproducible and can be divided out with a "blank" image.  However, we sometimes have to filter out the artifacts in Fourier space by masking with a thin horz. rectangle through the middle of the FFT map, save the very center.  Can someone please point out references where line artifacts have been removed with a filter (not by dividing out), whether in Fourier space or not?

 

Those interested can see sample images and FFT mask at http://www.mediafire.com/?sharekey=7b16b66ca9129ce991b20cc0d07ba4d2a83703a20834b252

 

Thanks,

Esteban

--
G. Esteban Fernandez, Ph.D.
Associate Director
Molecular Cytology Core Facility
University of Missouri
120 Bond Life Sciences Center
Columbia, MO  65211

http://www.biotech.missouri.edu/mcc/

573-882-4895
573-884-9395 fax

--

Zoltan Cseresnyes
Facility manager, Imaging Suite